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PMID: 6549046 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2316-20

Bradshaw HD, Deininger PL

Abstract

A cDNA containing the entire coding region of the human thymidine kinase gene has been molecularly cloned. The cDNA is under the control of a simian virus 40 promoter and is expressible in mammalian cells. The complete nucleotide sequence of the human thymidine kinase cDNA has been determined. The cDNA is 1,421 base pairs in length and has a large open reading frame of 702 base pairs capable of specifying a protein with a molecular weight of 25,504. Genomic Southern blotting experiments show that sequences homologous to the human thymidine kinase cDNA are conserved among many vertebrates, including prosimians (lemur), tree shrews, rats, mice, and chickens. Direct comparison of the nucleotide sequences of the human thymidine kinase cDNA and the chicken thymidine kinase gene reveals ca. 70% overall homology. This homology is extended further at the amino acid sequence level, with greater than 74% amino acid residues matched between the human and chicken thymidine kinase proteins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Genes Humans Species Specificity Thymidine Kinase/genetics Vertebrates/genetics
Chemicals
DNA Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bradshaw H D
Deininger P L
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2316-2320.1984
Published
1984-11-00
Pages
2316-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369060
Subset
IM
Grants
NIGMS NIH HHS · GM29848 · United States
NHLBI NIH HHS · HL07098 · United States
Databases
GENBANK
K02581
External Links
PubMed source DOI: 10.1128/mcb.4.11.2316-2320.1984 PMC Full Text View journal details
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