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PMID: 3138112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of chromosomal translocations to study human immunoglobulin gene organization: mapping DH segments within 35 kb of the C mu gene and identification of a new DH locus.

The EMBO journal ·Vol. 7 ·No. 7 ·1988-07-00 ·Pages 2003-10

Buluwela L, Albertson DG, Sherrington P, Rabbitts PH, Spurr N, Rabbitts TH

Abstract

We have studied the Burkitt's lymphoma cell line Daudi which carries the translocation t(8;14). The breakpoint of this translocation on the 14q+ chromosome occurs near to a rearranged DH-JH join, and the actual chromosome junction is a few hundred base pairs upstream of the joined DH element. The nucleotide sequence of the rearranged DH segment shows that it does not come from the previously described D cluster. Using this DH sequence as a probe we have identified two separate DH clusters. One of these is the major DH cluster and is located only 20 kb upstream of the JH segments. A pseudo-VH (probably the first VH segment) is also found approximately 98 kb from JH. A second, minor DH locus has been found which seems to be located on the distal side of the VH locus on chromosome 14, since there is little evidence for rearrangement or deletion of this locus in any B cell DNA analysed. A single VHIII subgroup gene is located within 25 kb of the newly identified DH element: it is possible that this minor locus occurs near the limit of the Igh locus.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Chromosomes, Human, Pair 14 Chromosomes, Human, Pair 8 Cloning, Molecular Cosmids Genes, Immunoglobulin Humans Immunoglobulin Heavy Chains/genetics Molecular Sequence Data Nucleic Acid Hybridization Translocation, Genetic
Chemicals
Immunoglobulin Heavy Chains
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Buluwela L
Medical Research Council Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.
Albertson D G
Sherrington P
Rabbitts P H
Spurr N
Rabbitts T H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-07-00
Pages
2003-10
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454475
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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