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PMID: 6329676 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of the hinge-coding segments in human immunoglobulin gamma heavy chain genes and the linkage of the gamma 2 and gamma 4 subclass genes.

The EMBO journal ·Vol. 1 ·No. 4 ·1982-00-00 ·Pages 403-7

Krawinkel U, Rabbitts TH

Abstract

The genes for the human immunoglobulin heavy chain constant region subclasses have been examined in clones isolated from a phage library containing human genomic DNA. Nucleotide sequencing and restriction enzyme mapping show that the CH1, hinge, CH2, and CH3 domains are present as distinct genetic elements separated by short intervening sequences in these genes: the gamma 3 gene is unique amongst these genes in that it possesses four separate hinge-coding segments. A further gamma gene has been identified which does not correspond to a known human gamma protein: this gene contains a hinge related to the first hinge of the gamma 3 gene and may represent an inactive or pseudo gamma gene. Comparison of a number of overlapping clones containing gamma 2 and gamma 4 genes shows that they are separated in human DNA by approximately 19 kb and that the gamma 2 gene is located upstream of gamma 4. Both genes (order 5'C gamma 2-C gamma 4-3') are orientated in the same direction of transcription.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genes Genetic Linkage Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin gamma-Chains/genetics Plasmids Protein Biosynthesis Structure-Activity Relationship Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin gamma-Chains DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krawinkel U
Rabbitts T H
References (37)
37 references, click to expand
  1. Ordering of mouse immunoglobulin heavy chain genes by molecular cloning.
    Nature. 1981 Jan 15;289(5794):149-53 PMID: 6779206
  2. Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6561-5 PMID: 6450418
  3. Cloning of human immunoglobulin mu gene and comparison with mouse mu gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5983-91 PMID: 6450943
  4. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  5. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  6. Human V kappa immunoglobulin gene number: implications for the origin of antibody diversity.
    Cell. 1981 Jun;24(3):613-23 PMID: 6265099
  7. Human immunoglobulin heavy chain genes: evolutionary comparisons of C mu, C delta and C gamma genes and associated switch sequences.
    Nucleic Acids Res. 1981 Sep 25;9(18):4509-24 PMID: 6795593
  8. Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain.
    Science. 1982 Apr 16;216(4543):309-11 PMID: 6801765
  9. Immunoglobulin heavy chain genes in humans are located on chromosome.
    Ann Hum Genet. 1981 Oct;45(Pt 4):331-5 PMID: 6805411
  10. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  11. Structural studies of immunoglobulin G.
    Nature. 1969 Jan 11;221(5176):145-8 PMID: 5782707
  12. The covalent structure of an entire gammaG immunoglobulin molecule.
    Proc Natl Acad Sci U S A. 1969 May;63(1):78-85 PMID: 5257969
  13. Human immunoglobulin subclasses. Partial amino acid sequence of the constant region of a gamma 4 chain.
    Biochem J. 1970 Mar;117(1):33-47 PMID: 4192699
  14. Human immunoglobulin E. The primary structure of the C-terminal domain of the epsilon chain.
    FEBS Lett. 1973 Jun 15;33(1):49-53 PMID: 4722495
  15. Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes.
    Adv Immunol. 1973;16:1-59 PMID: 4125921
  16. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  17. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  18. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  19. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  20. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  21. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  22. Studies of generation of B-cell diversity in mouse, man, and chicken.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:139-45 PMID: 408085
  23. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  24. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  27. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  28. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  29. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7 PMID: 283395
  30. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  31. Split immunoglobulin genes and human heavy chain deletion-mutants.
    J Immunol. 1979 Apr;122(4):1177-9 PMID: 109507
  32. A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.
    J Mol Biol. 1979 Mar 25;129(1):169-72 PMID: 448736
  33. Chromosomal location of the genes for human immunoglobulin heavy chains.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3416-9 PMID: 114999
  34. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene.
    Cell. 1979 Oct;18(2):559-68 PMID: 115593
  35. The variability, arrangement, and rearrangement of immunoglobulin genes.
    Can J Biochem. 1980 Mar;58(3):176-87 PMID: 6245773
  36. 5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains.
    Cell. 1980 Nov;22(1 Pt 1):187-96 PMID: 6775817
  37. A mouse immunoglobulin heavy chain deletion mutant: isolation of a cDNA clone and sequence analysis of the mRNA.
    Nucleic Acids Res. 1980 Apr 11;8(7):1475-84 PMID: 6776492
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
403-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553059
Subset
IM
Databases
GENBANK
J00230, K01313, K01316, K01320, V00554
External Links
PubMed source PMC Full Text View journal details
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