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PMID: 6776492 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mouse immunoglobulin heavy chain deletion mutant: isolation of a cDNA clone and sequence analysis of the mRNA.

Nucleic acids research ·Vol. 8 ·No. 7 ·1980-04-11 ·Pages 1475-84

Dunnick W, Rabbitts TH, Milstein C

Abstract

The mouse cell line IF2 secretes an immunoglobulin heavy chain lacking the CH1 domain. We have isolated and characterised a recombinant plasmid containing cDNA copies of the IF2 mutant mRNA. The cloned sequence extends from the nucleotides coding for amino acid 96 in the variable region through 100 nucleotides of untranslated region at the 3' end. The sequence of the cDNA insert reveals no discontinuity at the variable-hinge region junction, the site of the CH1 deletion. Experiments employing direct priming on the poly(A) tail of the IF2 heavy chain mRNA suggest that the 3' end of the cDNA clone (sequence C-C-C-T-G-C) is also the 3' end of the mRNA.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Cloning, Molecular DNA, Recombinant/metabolism Immunoglobulin Heavy Chains/genetics Mice Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Messenger/metabolism Transcription, Genetic
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dunnick W
Rabbitts T H
Milstein C
References (21)
21 references, click to expand
  1. A crude nuclease preparation suitable for use in DNA reassociation experiments.
    Biochim Biophys Acta. 1971 Jul 29;240(4):522-31 PMID: 5123563
  2. Human immunoglobulin E. The primary structure of the C-terminal domain of the epsilon chain.
    FEBS Lett. 1973 Jun 15;33(1):49-53 PMID: 4722495
  3. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):989-93 PMID: 165498
  4. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  5. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
    Nucleic Acids Res. 1976 Jan;3(1):101-16 PMID: 765970
  6. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  7. Amplification and characterization of a beta-globin gene synthesized in vitro.
    Cell. 1976 Jun;8(2):163-82 PMID: 61066
  8. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  9. Sequence analysis of the 3' non-coding regions of rabbit alpha- and beta-globin messenger RNAs.
    J Mol Biol. 1976 Nov 15;107(4):491-525 PMID: 63556
  10. Molecular analysis of spontaneous somatic mutants.
    Nature. 1977 Jan 27;265(5592):299-304 PMID: 401950
  11. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  12. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
  13. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370-4 PMID: 270678
  14. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  15. Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma gamma1 chain.
    J Biol Chem. 1978 Sep 10;253(17):6068-75 PMID: 98524
  16. Complete sequence of constant and 3' noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing.
    Cell. 1978 Nov;15(3):1067-75 PMID: 103625
  17. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  18. Immunoglobulin heavy chain gene organization in mice: analysis of a myeloma genomic clone containing variable and alpha constant regions.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):857-61 PMID: 106394
  19. A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.
    J Mol Biol. 1979 Mar 25;129(1):169-72 PMID: 448736
  20. Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain.
    Nucleic Acids Res. 1979 Jul 25;6(10):3305-21 PMID: 113776
  21. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene.
    Cell. 1979 Oct;18(2):559-68 PMID: 115593
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.7.1475
Published
1980-04-11
Pages
1475-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324010
Subset
IM
Databases
GENBANK
J00543, K00685, K00686, N00006, N00007
External Links
PubMed source DOI: 10.1093/nar/8.7.1475 PMC Full Text View journal details
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