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PMID: 315514 Published · ppublish English Journal Article

Surface glycoproteins of resting and activated human T lymphocytes.

Molecular and cellular biochemistry ·Vol. 27 ·No. 2 ·1979-10-15 ·Pages 117-31

Andersson LC, Gahmberg CG

Abstract

This review summarizes some recent studies on the surface glycoproteins of human thymocytes and T lymphocytes. Purified cells were surface labeled by the galactose oxidase-NaB3H4 or periodate-NaB3H4 techniques. The radioactive membrane glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by fluorography. Thymocytes and T lymphocytes show characteristic surface glycoprotein profiles which are easily distinguishable from those of the other main groups of human leukocytes. We observed specific changes in the surface glycoprotein patterns which correlate with the degree of maturation and functional activation of T cells. Surface molecules carrying T cell specific antigens were identified by immune-precipitation from lysates of surface labeled thymocytes and T lymphocytes using rabbit anti-human T cell antibodies. Finally we describe a leukocyte membrane glycoprotein which is a precursor of serum alpha 1 acid glycoprotein (orosomucoid).

MeSH Terms
Antigens, Surface/analysis B-Lymphocytes/immunology Binding Sites, Antibody Cell Membrane/immunology Electrophoresis, Polyacrylamide Gel Glycoproteins/blood Humans Infant Isotope Labeling/methods Membrane Proteins/blood Orosomucoid/blood T-Lymphocytes/immunology
Chemicals
Antigens, Surface Glycoproteins Membrane Proteins Orosomucoid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Andersson L C
Gahmberg C G
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00218355
Published
1979-10-15
Pages
117-31
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00218355 View journal details
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