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PMID: 3174433 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Human liver-type arginase gene: structure of the gene and analysis of the promoter region.

Nucleic acids research ·Vol. 16 ·No. 18 ·1988-09-26 ·Pages 8789-802

Takiguchi M, Haraguchi Y, Mori M

Abstract

The gene for human liver-type arginase (EC 3.5.3.1), a urea cycle enzyme, was cloned and the structure was determined. This gene is 11.5 kilobases long and is split into 8 exons. The cap site was determined by nuclease S1 mapping and primer extension. A "TATA box"-like sequence is located 28 bases upstream from the cap site, and a sequence similar to the binding sites of the transcription factor CTF/NF1, a "CAAT box"-binding protein, is located 72 bases upstream. In the 5' end region, sequences resembling the glucocorticoid responsive elements, the cAMP responsive elements, and the enhancer core sequences are present. The immediately 5' flanking region of the human gene up to position -105 is 84% identical with the corresponding segment of the rat gene. In this region of the human gene, one DNase I-protected area and several hypersensitive cleavage sites were detected by footprint analysis, using nuclear extracts from the rat liver. The protected area contains the sequence similar to the binding sites of CTF/NF1 and also overlaps with the sequence resembling the glucocorticoid responsive elements.

MeSH Terms
Amino Acid Sequence Animals Arginase/genetics Base Sequence Cloning, Molecular Deoxyribonuclease I/metabolism Genes Humans Introns Liver/enzymology Molecular Sequence Data Promoter Regions, Genetic Rats Regulatory Sequences, Nucleic Acid Restriction Mapping
Chemicals
Deoxyribonuclease I Arginase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takiguchi M
Institute for Medical Genetics, Kumamoto University Medical School, Japan.
Haraguchi Y
Mori M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.18.8789
Published
1988-09-26
Pages
8789-802
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338635
Subset
IM
Databases
GENBANK
X12662, X12663, X12664, X12665, X12666, X12667, X12668, X12669
External Links
PubMed source DOI: 10.1093/nar/16.18.8789 PMC Full Text View journal details
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