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PMID: 3205720 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5.

Nucleic acids research ·Vol. 16 ·No. 22 ·1988-11-25 ·Pages 10669-79

Dutreix M, Bäckman A, Célérier J, Bagdasarian MM, Sommer S, Bailone A, Devoret R, Bagdasarian M

Abstract

PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes share extensive homology the predicted amino acid sequences of the two proteins differing by 5 residues, (ii) the expression of R6-5 psiB is 4 times higher than F psiB gene, (iii) in plasmid R6-5, a Tn10 transposon upstream from the psiB gene enhances psiB expression. Hence, the F sex factor may be unable to prevent SOS induction for two non-exclusive reasons: (i) F PsiB protein, being slightly different from R6-5, may be less active, (ii) the level of synthesis of F PsiB protein may be insufficient to prevent SOS induction.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence F Factor Genes Genes, Bacterial Molecular Sequence Data R Factors Recombinant Fusion Proteins/isolation & purification Recombinant Proteins/isolation & purification Restriction Mapping
Chemicals
Bacterial Proteins PsiB protein, Bacteria Recombinant Fusion Proteins Recombinant Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dutreix M
GEMC, Enzymologie, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Bäckman A
Célérier J
Bagdasarian M M
Sommer S
Bailone A
Devoret R
Bagdasarian M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.22.10669
Published
1988-11-25
Pages
10669-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338932
Subset
IM
Databases
GENBANK
X12462, X12463
External Links
PubMed source DOI: 10.1093/nar/16.22.10669 PMC Full Text View journal details
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