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PMID: 3219917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterochromatic DNA in Triturus (Amphibia, Urodela) II. A centromeric satellite DNA.

Chromosoma ·Vol. 97 ·No. 3 ·1988-11-00 ·Pages 204-11

Cremisi F, Vignali R, Batistoni R, Barsacchi G

Abstract

The MspI family of highly repeated sequences is a centromeric satellite DNA representing about 1% of the genome of the Italian smooth newt, Triturus vulgaris meridionalis. We have studied the structure, genomic organization, chromosomal localization and conservation across species of this family. MspI sequences are around 197 bp long, as shown by sequencing of three cloned units. The family is organized in large clusters of tandemly arrayed units, present at almost all the centromeres of T.v. meridionalis, and is well conserved in the T.v. vulgaris subspecies. Conserved MspI sequences are also present in the related species T. helveticus, where they appear to be clustered at the centromeres of only a few chromosomes. MspI sequences are not found in other Triturus species analysed. The correlation of these sequences with the overall distribution pattern of heterochromatin and the extent of their conservation within the genus Triturus, are discussed.

MeSH Terms
Animals Blotting, Southern Centromere Chromosomes Cloning, Molecular DNA, Satellite/genetics,isolation & purification Heterochromatin/genetics Immunoblotting Karyotyping Molecular Sequence Data Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Restriction Mapping Triturus/genetics
Chemicals
DNA, Satellite Heterochromatin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cremisi F
Dipartimento di Fisiologia e Biochimica, Laboratori di Biologia Cellulare e dello Sviluppo, Pisa, Italy.
Vignali R
Batistoni R
Barsacchi G
References (34)
34 references, click to expand
  1. Rapid and concerted evolution of repeat units in a balbiani ring gene.
    Genetics. 1987 Sep;117(1):43-9 PMID: 17246397
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.
    Mol Cell Biol. 1987 Jan;7(1):403-9 PMID: 3550420
  4. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  5. Modes and rates of change of complex DNA families of Drosophila.
    J Mol Biol. 1982 Jun 15;158(1):37-54 PMID: 6811756
  6. In situ hybridization of highly repetitive DNA to chromosomes of Triturus cristatus.
    Chromosoma. 1979 Feb 13;71(1):57-64 PMID: 428272
  7. Self-cleaving transcripts of satellite DNA from the newt.
    Cell. 1987 Feb 13;48(3):535-43 PMID: 2433049
  8. Evolution of highly repeated DNA within the genusTriturus (Amphibia, Urodela).
    Cytotechnology. 1988 Feb;1(2):185-8 PMID: 22358572
  9. The maps of the lampbrush chromosomes of Triturus (Amphibia Urodela). IV. Triturus vulgaris meridionalis.
    Chromosoma. 1970;31(3):255-79 PMID: 5489360
  10. Repeated DNA still in search of a function.
    Science. 1982 Aug 13;217(4560):621-3 PMID: 6283639
  11. Satellite DNA is transcribed on lampbrush chromosomes.
    Nature. 1980 Feb 14;283(5748):686-8 PMID: 7188786
  12. Banding patterns in newt chromosomes by the Giemsa stain.
    Chromosoma. 1973;40(4):321-31 PMID: 4120883
  13. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
    Anal Biochem. 1983 Jul 1;132(1):14-9 PMID: 6312834
  14. Molecular drive: a cohesive mode of species evolution.
    Nature. 1982 Sep 9;299(5879):111-7 PMID: 7110332
  15. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  16. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  17. Functional selection and analysis of yeast centromeric DNA.
    Cell. 1985 Oct;42(3):913-21 PMID: 2996783
  18. Heterochromatic DNA in Triturus (Amphibia, Urodela). I. A satellite DNA component of the pericentric C-bands.
    Chromosoma. 1986;93(5):435-46 PMID: 3013516
  19. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  20. Cytological evidence of transcription of highly repeated DNA sequences during the lampbrush stage in Triturus cristatus carnifex.
    Chromosoma. 1980;80(3):289-307 PMID: 6160022
  21. Molecular evidence for genetic exchanges among ribosomal genes on nonhomologous chromosomes in man and apes.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7323-7 PMID: 6261251
  22. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  23. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  24. Histone genes are located at the sphere loci of newt lampbrush chromosomes.
    Chromosoma. 1981;84(2):159-71 PMID: 7327042
  25. Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus.
    Cell. 1981 Jun;24(3):649-59 PMID: 6166385
  26. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  27. Centromeric satellite DNA in the newt Triturus cristatus karelinii and related species: its distribution and transcription on lampbrush chromosomes.
    Chromosoma. 1985;92(2):100-7 PMID: 2988877
  28. Fine structure and evolution of DNA in heterochromatin.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1121-35 PMID: 98264
  29. Human nucleolus organizers on nonhomologous chromosomes can share the same ribosomal gene variants.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5744-8 PMID: 6272316
  30. Transcription of a satellite DNA in the newt.
    J Cell Biol. 1986 Oct;103(4):1137-44 PMID: 3771629
  31. Dynamics of concerted evolution of ribosomal DNA and histone gene families in the melanogaster species subgroup of Drosophila.
    J Mol Biol. 1982 Jun 15;158(1):17-35 PMID: 6811755
  32. Structural characteristics of genome organization in amphibians: differential staining of chromosomes and DNA structure.
    J Mol Evol. 1982;18(2):73-91 PMID: 7047753
  33. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  34. The biological significance of variation in satellite DNA and heterochromatin in newts of the genus Triturus: an evolutionary perspective.
    Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):243-59 PMID: 2870520
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292962
Published
1988-11-00
Pages
204-11
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292962 View journal details
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