Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.

Journal of clinical microbiology ·Vol. 5 ·No. 3 ·1977-03-00 ·Pages 273-7

Walls KW, Bullock SL, English DK

Abstract

The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the microtitration method were used successfully. ELISA results are equivalent to those found in the indirect immunofluorescence test, yet the ELISA procedure is simpler and more rapid to perform.

MeSH Terms

Diagnosis, Differential Enzyme-Linked Immunosorbent Assay/methods Evaluation Studies as Topic Fluorescent Antibody Technique Humans Immunoenzyme Techniques/methods Toxoplasmosis/diagnosis

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Walls K W

Bullock S L

English D K

References (12)

12 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.5.3.273-277.1977

Published

1977-03-00

Pages

273-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274581

Subset

External Links

PubMed source DOI: 10.1128/jcm.5.3.273-277.1977 PMC Full Text View journal details

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Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.

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