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PMID: 3244358 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulatory myosin light-chain genes of Caenorhabditis elegans.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5339-49

Cummins C, Anderson P

Abstract

We have cloned and analyzed the Caenorhabditis elegans regulatory myosin light-chain genes. C. elegans contains two such genes, which we have designated mlc-1 and mlc-2. The two genes are separated by 2.6 kilobases and are divergently transcribed. We determined the complete nucleotide sequences of both mlc-1 and mlc-2. A single, conservative amino acid substitution distinguishes the sequences of the two proteins. The C. elegans proteins are strongly homologous to regulatory myosin light chains of Drosophila melanogaster and vertebrates and weakly homologous to a superfamily of eucaryotic calcium-binding proteins. Both mlc-1 and mlc-2 encode abundant mRNAs. We mapped the 5' termini of these transcripts by using primer extension sequencing of mRNA templates. mlc-1 mRNAs initiate within conserved hexanucleotides at two different positions, located at -28 and -38 relative to the start of translation. The 5' terminus of mlc-2 mRNA is not encoded in the 4.8-kilobase genomic region upstream of mlc-2. Rather, mlc-2 mRNA contains at its 5' end a short, untranslated leader sequence that is identical to the trans-spliced leader sequence of three C. elegans actin genes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caenorhabditis/genetics Cloning, Molecular Genes Genes, Regulator Molecular Sequence Data Myosin Subfragments Myosins/genetics Peptide Fragments/genetics Plasmids Restriction Mapping Transcription, Genetic
Chemicals
Myosin Subfragments Peptide Fragments Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cummins C
Department of Genetics, University of Wisconsin, Madison 53706.
Anderson P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5339-5349.1988
Published
1988-12-00
Pages
5339-49
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365636
Subset
IM
Grants
NIGMS NIH HHS · GM30132 · United States
Databases
GENBANK
M23365, M23366
External Links
PubMed source DOI: 10.1128/mcb.8.12.5339-5349.1988 PMC Full Text View journal details
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