Home LiteratureArticle Details
PMID: 3278300 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutational analysis of the tobacco mosaic virus 5'-leader for altered ability to enhance translation.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 883-93

Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TM

Abstract

Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in omega's ability to enhance expression of beta-glucuronidase (GUS) mRNA in tobacco mesophyll protoplasts and Escherichia coli or chloramphenicol acetyltransferase (CAT) mRNA in Xenopus laevis oocytes. Alteration of an eight base subsequence required for the binding of a second ribosome resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the mutants in E. coli.

MeSH Terms
Animals Escherichia coli/metabolism Mutation Oocytes/metabolism Plants, Toxic Protein Biosynthesis Protoplasts/metabolism Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid Tobacco/metabolism Tobacco Mosaic Virus/genetics Xenopus laevis
Chemicals
Recombinant Fusion Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gallie D R
Department of Virus Research, John Innes Institute, AFRC Institute of Plant Science Research, Norwich, UK.
Sleat D E
Watts J W
Turner P C
Wilson T M
References (21)
21 references, click to expand
  1. Binding of ribosomes to the 5' leader sequence (N = 258) of RNA 3 from alfalfa mosaic virus.
    Nucleic Acids Res. 1981 Mar 11;9(5):1087-100 PMID: 7232211
  2. Genetic map of the crown gall suppressive IncW plasmid pSa.
    Mol Gen Genet. 1982;186(1):10-5 PMID: 6287164
  3. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  4. Nucleotide sequence of tobacco mosaic virus RNA.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818-22 PMID: 6964389
  5. Cross-linking of tobacco mosaic virus RNA and capped polyribonucleotides to 18S rRNA in wheat germ ribosome-mRNA complexes.
    J Biochem. 1983 Dec;94(6):1803-8 PMID: 6671966
  6. Multiple ribosome binding to the 5'-terminal leader sequence of tobacco mosaic virus RNA. Assembly of an 80S ribosome X mRNA complex at the AUU codon.
    Eur J Biochem. 1984 May 2;140(3):503-11 PMID: 6723646
  7. Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.
    Nucleic Acids Res. 1984 Jun 11;12(11):4539-57 PMID: 6547529
  8. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  9. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  10. Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence.
    Nature. 1987 Feb 12-18;325(6105):622-5 PMID: 3492677
  11. The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo.
    Nucleic Acids Res. 1987 Apr 24;15(8):3257-73 PMID: 3575095
  12. In vivo uncoating and efficient expression of foreign mRNAs packaged in TMV-like particles.
    Science. 1987 May 29;236(4805):1122-4 PMID: 3472350
  13. The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6030-4 PMID: 3476924
  14. A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo.
    Nucleic Acids Res. 1987 Nov 11;15(21):8693-711 PMID: 2825117
  15. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  16. Messenger RNA for the coat protein of tobacco mosaic virus.
    Nature. 1976 Apr 29;260(5554):759-64 PMID: 1264250
  17. In vitro, the major ribosome binding site on Rous sarcoma virus RNA does not contain the nucleotide sequence coding for the N-terminal amino acids of the gag gene product.
    J Virol. 1979 Feb;29(2):597-611 PMID: 219249
  18. Characterization of long guanosine-free RNA sequences from the Dahlemense and U2 strains of tobacco mosaic virus.
    Eur J Biochem. 1979 Jul;98(1):61-6 PMID: 111935
  19. Binding of ribosomes to 5'-terminal leader sequences of eukaryotic messenger RNAs.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3111-5 PMID: 290990
  20. Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3.
    Nature. 1979 Sep 27;281(5729):277-82 PMID: 551276
  21. Binding of ribosomes to linear and circular forms of the 5'-terminal leader fragment of tobacco-mosaic-virus RNA.
    Eur J Biochem. 1981 Feb;114(2):221-7 PMID: 6783406
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.3.883
Published
1988-02-11
Pages
883-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334725
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.3.883 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2