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PMID: 3283703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P from Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 16 ·No. 6 ·1988-03-25 ·Pages 2537-52

Leontis N, DaLio A, Strobel M, Engelke D

Abstract

RNase P derived from S. cerevisiae nuclei was tested for its ability to cleave a variety of naturally occurring and selectively altered precursor-tRNA molecules to yield matured 5' termini. Precursors were synthesized in vitro in order to test which aspects of substrate structure are crucial to recognition and cleavage by RNase P. Base modifications in the precursor substrates are not required for cleavage by the enzyme, but deletion and substitution mutations affecting any portion of the precursor tertiary structure reduce cleavage. In particular, a number of alterations in the intervening sequence (IVS) reduce the susceptibility of the substrate to cleavage by RNase P. The significance of these results is discussed in reference to the contribution of the IVS to the structure of the precursor-tRNA.

MeSH Terms
DNA Mutational Analysis Endoribonucleases/metabolism Introns Nucleic Acid Conformation RNA Processing, Post-Transcriptional RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Leu/genetics Ribonuclease P Saccharomyces cerevisiae/enzymology Suppression, Genetic
Chemicals
RNA, Transfer, Amino Acid-Specific RNA, Transfer, Leu Endoribonucleases Ribonuclease P
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leontis N
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109.
DaLio A
Strobel M
Engelke D
References (39)
39 references, click to expand
  1. Characterization of the yeast mitochondrial locus necessary for tRNA biosynthesis: DNA sequence analysis and identification of a new transcript.
    Cell. 1983 Oct;34(3):911-7 PMID: 6313214
  2. A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter.
    Cell. 1983 Jun;33(2):585-93 PMID: 6552939
  3. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.
    Cell. 1983 Dec;35(3 Pt 2):849-57 PMID: 6197186
  4. Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
    J Biol Chem. 1984 Apr 25;259(8):5197-207 PMID: 6371001
  5. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  6. Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.
    Nucleic Acids Res. 1984 Dec 21;12(24):9367-82 PMID: 6096826
  7. Nucleolytic processing of a tRNAArg-tRNAAsp dimeric precursor by a homologous component from Saccharomyces cerevisiae.
    J Biol Chem. 1985 Jan 25;260(2):1271-9 PMID: 2981839
  8. Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.
    J Biol Chem. 1985 Mar 10;260(5):3108-15 PMID: 3882706
  9. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.
    Mol Cell Biol. 1985 Apr;5(4):808-15 PMID: 3921825
  10. Characterization of an RNase P activity from HeLa cell mitochondria. Comparison with the cytosol RNase P activity.
    J Biol Chem. 1985 May 25;260(10):5942-9 PMID: 2581945
  11. A single base change in the intron of a serine tRNA affects the rate of RNase P cleavage in vitro and suppressor activity in vivo in Saccharomyces cerevisiae.
    J Biol Chem. 1986 May 5;261(13):5878-85 PMID: 3516987
  12. Eukaryotic pre-tRNA 5' processing nuclease: copurification with a complex cylindrical particle.
    Cell. 1986 Aug 1;46(3):377-85 PMID: 3637121
  13. Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.
    EMBO J. 1986 Jul;5(7):1697-703 PMID: 3743551
  14. Direct identification of small sequence changes in chromosomal DNA.
    Gene. 1986;44(1):151-8 PMID: 2429900
  15. RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components.
    Mol Cell Biol. 1986 Apr;6(4):1058-64 PMID: 3537697
  16. Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.
    Mol Cell Biol. 1986 Jul;6(7):2663-73 PMID: 3537724
  17. Intron mutations affect splicing of Saccharomyces cerevisiae SUP53 precursor tRNA.
    Mol Cell Biol. 1986 Jul;6(7):2674-83 PMID: 3537725
  18. Nucleotide modification in vitro of the precursor of transfer RNA of Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3626-30 PMID: 4587257
  19. Identification of a ribonuclease P-like activity from human KB cells.
    Cell. 1976 Sep;9(1):101-16 PMID: 10085
  20. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  21. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  22. A yeast mutant which accumulates precursor tRNAs.
    Cell. 1978 Jun;14(2):211-9 PMID: 352536
  23. Transcription and processing of intervening sequences in yeast tRNA genes.
    Cell. 1978 Jun;14(2):221-36 PMID: 352537
  24. Ribonuclease P: an enzyme with an essential RNA component.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3717-21 PMID: 358197
  25. E. coli RNAase P has a required RNA component.
    Cell. 1980 Apr;19(4):881-7 PMID: 6155215
  26. Assembly of the mitochondrial membrane system. Structure and location of the mitochondrial glutamic tRNA gene in Saccharomyces cerevisiae.
    FEBS Lett. 1980 Apr 21;113(1):52-4 PMID: 6247202
  27. RNase P of Bacillus subtilis has a RNA component.
    J Biol Chem. 1980 Aug 25;255(16):7507-9 PMID: 6156938
  28. Directed deletion of a yeast transfer RNA intervening sequence.
    Science. 1980 Sep 19;209(4463):1396-400 PMID: 6997991
  29. Veal heart ribonuclease P has an essential RNA component.
    Biochem Biophys Res Commun. 1980 Sep 30;96(2):831-7 PMID: 6158945
  30. Recognition of local nucleotide conformation in contrast to sequence by a rRNA processing endonuclease.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5644-8 PMID: 6777771
  31. Identification of ribonuclease P activity from chick embryos.
    Biochim Biophys Acta. 1980 Jun 13;613(2):439-47 PMID: 6160876
  32. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  33. Properties of purified ribonuclease P from Escherichia coli.
    Biochemistry. 1981 Mar 31;20(7):1902-6 PMID: 6164392
  34. Partial purification of RNase P from Schizosaccharomyces pombe.
    J Biol Chem. 1981 May 25;256(10):5058-63 PMID: 6262315
  35. Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.
    J Biol Chem. 1982 May 25;257(10):5772-8 PMID: 7040372
  36. Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.
    Cell. 1983 Jan;32(1):67-76 PMID: 6337723
  37. A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P.
    Nucleic Acids Res. 1983 Mar 11;11(5):1491-505 PMID: 6298744
  38. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product.
    Nature. 1983 Apr 21;302(5910):681-7 PMID: 6339954
  39. tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter.
    Cell. 1983 Nov;35(1):117-25 PMID: 6226365
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.6.2537
Published
1988-03-25
Pages
2537-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336388
Subset
IM
Grants
NIGMS NIH HHS · R01 GM34869 · United States
External Links
PubMed source DOI: 10.1093/nar/16.6.2537 PMC Full Text View journal details
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