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PMID: 3921825 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.

Molecular and cellular biology ·Vol. 5 ·No. 4 ·1985-04-00 ·Pages 808-15

Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D

Abstract

Suppression of nonsense codons in Schizosaccharomyces pombe by sup3-e tRNASerUGA or sup3-i tRNASerUAA is reduced or abolished by mutations within the suppressor locus. Twenty-five suppressor-inactive sup3-e genes and thirteen mutant sup3-i genes were isolated from S. pombe genomic clone banks by colony hybridization. Sequence analysis of these revertant alleles corroborates genetic evidence for mutational hotspots within the sup3 tRNA gene. Fifteen types of point mutations or insertions were found. Many of these replace bases which are highly or completely conserved in eucaryotic tRNA genes. Transcription of the altered sup3 genes in a Saccharomyces cerevisiae extract enabled the identification of mutations which affect the rate of 5'-end maturation or splicing of the tRNA precursors or both. A total of seven mutations were found which alter transcriptional efficiencies. Of these, five are located outside the internal transcription control regions.

MeSH Terms
Ascomycota/genetics Base Sequence Cloning, Molecular Gene Expression Regulation Genes Genes, Fungal Mutation RNA Processing, Post-Transcriptional RNA Splicing RNA, Transfer/genetics Schizosaccharomyces/genetics Serine Suppression, Genetic Templates, Genetic Transcription, Genetic
Chemicals
Serine RNA, Transfer
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pearson D
Willis I
Hottinger H
Bell J
Kumar A
Leupold U
Söll D
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.4.808-815.1985
Published
1985-04-00
Pages
808-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366785
Subset
IM
Databases
GENBANK
M10890
External Links
PubMed source DOI: 10.1128/mcb.5.4.808-815.1985 PMC Full Text View journal details
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