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PMID: 3292537 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast vacuoles fragment when microtubules are disrupted.

The Journal of cell biology ·Vol. 107 ·No. 1 ·1988-07-00 ·Pages 115-20

Guthrie BA, Wickner W

Abstract

To identify whether microtubules are involved in the maintenance of vacuolar morphology, we treated Saccharomyces cerevisiae with nocodazole and methyl benzimidazole-2-yl-carbamate, drugs which inhibit the polymerization of microtubules. Treated cells arrest with a single large bud in the G2/prophase portion of the cell cycle. Labeling the vacuole with either quinacrine or FITC-dextran revealed vacuole fragmentation that was not found in untreated cells or in cells arrested in G2 by unrelated means. A drug-resistant mutant in beta tubulin does not show vacuolar fragmentation when treated with drug. We propose that microtubules are involved in the regulation of vacuole morphology.

MeSH Terms
Antifungal Agents/pharmacology Benzimidazoles/pharmacology Fluorescent Antibody Technique Interphase Microtubules/drug effects,physiology,ultrastructure Nocodazole Organoids/ultrastructure Prophase Saccharomyces cerevisiae/cytology,drug effects,ultrastructure Vacuoles/drug effects,ultrastructure
Chemicals
Antifungal Agents Benzimidazoles Nocodazole
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guthrie B A
Molecular Biology Institute, University of California, Los Angeles 90024.
Wickner W
References (30)
30 references, click to expand
  1. Tubular lysosome morphology and distribution within macrophages depend on the integrity of cytoplasmic microtubules.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1921-5 PMID: 3550801
  2. Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins.
    Mol Cell Biol. 1986 Nov;6(11):3722-33 PMID: 3540600
  3. Apparent endocytosis of fluorescein isothiocyanate-conjugated dextran by Saccharomyces cerevisiae reflects uptake of low molecular weight impurities, not dextran.
    J Cell Biol. 1987 Nov;105(5):1981-7 PMID: 2445758
  4. Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip.
    J Biophys Biochem Cytol. 1960 Feb;7:167-80 PMID: 14434278
  5. Studies on secretion and endocytosis of macromolecules by cultivated skin fibroblasts. Effects of anti-microtubular agents on secretion and endocytosis of lysosomal hydrolases and of sulphated glycosaminoglycans.
    Biochem J. 1978 Feb 15;170(2):313-20 PMID: 637845
  6. Fidelity of conjugation in Saccharomyces cerevisiae.
    Mol Gen Genet. 1978 Jun 14;162(2):173-82 PMID: 27718
  7. Mitosis in rat thyroid epithelial cells in vivo. I. Ultrastructural changes in cytoplasmic organelles during the mitotic cycle.
    J Ultrastruct Res. 1979 Jan;66(1):53-77 PMID: 423323
  8. The nuclear envelope lamina is reversibly depolymerized during mitosis.
    Cell. 1980 Jan;19(1):277-87 PMID: 7357605
  9. Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae.
    J Cell Biol. 1980 Jul;86(1):123-8 PMID: 6998984
  10. The influence of the microtubule inhibitor, methyl benzimidazol-2-yl-carbamate (MBC) on nuclear division and the cell cycle in Saccharomyces cerevisiae.
    J Cell Sci. 1980 Dec;46:341-52 PMID: 7014573
  11. A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    J Cell Biol. 1982 Jan;92(1):108-12 PMID: 6120174
  12. Lethal disruption of the yeast actin gene by integrative DNA transformation.
    Science. 1982 Jul 23;217(4557):371-3 PMID: 7046050
  13. Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase.
    Exp Cell Res. 1982 Nov;142(1):85-94 PMID: 6814941
  14. The partitioning of cytoplasmic organelles at cell division.
    Int Rev Cytol Suppl. 1983;15:49-89 PMID: 6343284
  15. Benomyl prevents nuclear fusion in Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;193(1):188-9 PMID: 6361497
  16. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  17. A monoclonal antibody against a 135-K Golgi membrane protein.
    EMBO J. 1982;1(12):1621-8 PMID: 7188254
  18. Interaction of rat liver lysosomal membranes with actin.
    J Cell Biol. 1984 Aug;99(2):680-5 PMID: 6746742
  19. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
    Cell. 1983 May;33(1):211-9 PMID: 6380751
  20. Associations of elements of the Golgi apparatus with microtubules.
    J Cell Biol. 1984 Sep;99(3):1092-100 PMID: 6381504
  21. Antibodies against lysosomal membranes reveal a 100,000-mol-wt protein that cross-reacts with purified H+,K+ ATPase from gastric mucosa.
    J Cell Biol. 1984 Oct;99(4 Pt 1):1511-26 PMID: 6207183
  22. Tubulin dynamics in cultured mammalian cells.
    J Cell Biol. 1984 Dec;99(6):2175-86 PMID: 6501419
  23. In vitro translocation of organelles along microtubules.
    Cell. 1985 Apr;40(4):729-30 PMID: 2580633
  24. A vital stain for the Golgi apparatus.
    Science. 1985 May 10;228(4700):745-7 PMID: 2581316
  25. Identification of myosin heavy chain in Saccharomyces cerevisiae.
    Nature. 1985 Jul 4-10;316(6023):83-5 PMID: 3892306
  26. Endocytosis in Saccharomyces cerevisiae: internalization of alpha-amylase and fluorescent dextran into cells.
    EMBO J. 1985 Jul;4(7):1861-6 PMID: 2411550
  27. Isolation and characterization of mutations in the beta-tubulin gene of Saccharomyces cerevisiae.
    Genetics. 1985 Dec;111(4):715-34 PMID: 2998923
  28. Components of microtubular structures in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2468-72 PMID: 3517870
  29. Immunofluorescence of the microtubular skeleton in growing and drug-treated yeast protoplasts.
    Eur J Cell Biol. 1986 Aug;41(2):150-6 PMID: 3530761
  30. Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.
    J Cell Biol. 1987 Oct;105(4):1539-47 PMID: 2444598
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.1.115
Published
1988-07-00
Pages
115-20
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115174
Subset
IM
Grants
NIGMS NIH HHS · GM-07104 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.1.115 PMC Full Text View journal details
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