Home LiteratureArticle Details
PMID: 331253 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A restriction endonuclease cleavage map of mouse mitochondrial DNA.

Nucleic acids research ·Vol. 4 ·No. 5 ·1977-00-00 ·Pages 1273-89

Moore KH, Johnson PH, Chandler SE, Grossman LI

Abstract

A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonucleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI. The migration of linear DNA with a molecular weight greater than 1 X 10(6) was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.

MeSH Terms
Animals Bacillus/enzymology Cell Line Coliphages DNA Polymerase I DNA Restriction Enzymes DNA, Circular DNA, Mitochondrial DNA, Viral Endonucleases Escherichia coli/enzymology Haemophilus influenzae/enzymology Mice Microscopy, Electron Molecular Weight
Chemicals
DNA, Circular DNA, Mitochondrial DNA, Viral DNA Polymerase I Endonucleases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Moore K H
Johnson P H
Chandler S E
Grossman L I
References (35)
35 references, click to expand
  1. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:251-8 PMID: 14114851
  2. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.
    J Biol Chem. 1964 Jan;239:222-32 PMID: 14114848
  3. Complex forms and replicative intermediates of mitochondrial DNA in tissues from adult and senescent mice.
    Nucleic Acids Res. 1977;4(5):1301-14 PMID: 896462
  4. Structural heterogeneity of mitochondrial DNA molecules within the genus Drosophila.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3623-7 PMID: 1068475
  5. Mechanism of mitochondrial DNA replication in mouse L-cells: asynchronous replication of strands, segregation of circular daughter molecules, aspects of topology and turnover of an initiation sequence.
    J Mol Biol. 1974 Jul 15;86(4):801-24 PMID: 4473554
  6. Restriction endonuclease cleavage maps of animal mitochondrial DNAs.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4617-21 PMID: 4216020
  7. Cleavage of replicating forms of mitochondrial DNA by EcoRI endonuclease.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4447-51 PMID: 4612520
  8. Structure of an intermediate in the replication of bacteriophage phiX174 deoxyribonucleic acid: the initiation site for DNA replication.
    J Mol Biol. 1974 Feb 15;83(1):47-61 PMID: 4594147
  9. Replication of mitochondrial DNA. Circular replicative intermediates in mouse L cells.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):737-41 PMID: 4501588
  10. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252-7 PMID: 5289384
  11. Replication of mitochondrial DNA in mouse L cells and their thymidine kinase - derivatives: displacement replication on a covalently-closed circular template.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3810-4 PMID: 4509344
  12. Pulse-labeled components in the replication of mitochondrial deoxyribonucleic acid.
    J Biol Chem. 1973 Jun 25;248(12):4512-4 PMID: 4736430
  13. Unidirectionality of replication in mouse mitochondrial DNA.
    Nat New Biol. 1973 Jan 24;241(108):103-5 PMID: 4512449
  14. Role of base composition in the electrophoresis of microbial and crab DNA in polyacrylamide gels.
    Nat New Biol. 1972 Jul 19;238(81):65-9 PMID: 4625705
  15. Replication of mitochondrial DNA: replicative forms of molecules from rat tissues and evidence for discontinuous replication.
    Cold Spring Harb Symp Quant Biol. 1974;38:267-80 PMID: 4364784
  16. In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture.
    J Mol Biol. 1971 Jul 28;59(2):255-72 PMID: 4327779
  17. Mechanism of mitochondrial DNA replication in mouse L-cells: topology of circular daughter molecules and dynamics of catenated oligomer formation.
    J Mol Biol. 1976 Jan 5;100(1):85-92 PMID: 1249842
  18. Replication of circular DNA in eukaryotic cells.
    Annu Rev Biochem. 1974;43(0):695-719 PMID: 4605048
  19. An electron microscope study of Sindbis virus RNA.
    Cold Spring Harb Symp Quant Biol. 1974;38:943-50 PMID: 4598643
  20. Complex mitochondrial DNA in human tumors.
    Cancer Res. 1973 May;33(5):1065-70 PMID: 4512952
  21. The replication and structure of mitochondrial DNA in animal cells.
    Cold Spring Harb Symp Quant Biol. 1974;38:281-8 PMID: 4524759
  22. Complex mitochondrial DNA.
    Int Rev Exp Pathol. 1975;14:1-67 PMID: 170231
  23. A rapid method for desalting small volumes of solution.
    Anal Biochem. 1973 Sep;55(1):328-30 PMID: 4753159
  24. 5-bromodeoxyuridine labeling of monomeric and catenated circular mitochondrial DNA in HeLa cells.
    J Mol Biol. 1973 Feb 25;74(2):81-94 PMID: 4689958
  25. Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP.
    J Virol. 1973 Apr;11(4):596-9 PMID: 4700513
  26. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.
    J Mol Biol. 1972 Feb 14;63(3):383-95 PMID: 5014925
  27. The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12.
    Cell. 1976 Apr;7(4):517-30 PMID: 782720
  28. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  29. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
    J Mol Biol. 1975 Sep 5;97(1):123-5 PMID: 1177312
  30. Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes.
    FEBS Lett. 1975 Apr 15;53(1):84-6 PMID: 1140401
  31. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  32. Complex mitochondrial DNA in leukemic and normal human myeloid cells.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1077-84 PMID: 5256408
  33. Sedimentation velocity properties of complex mitochondrial DNA.
    Nature. 1969 Jan 25;221(5178):332-7 PMID: 5764435
  34. Occurrence of complex mitochondrial DNA in normal tissues.
    Nature. 1968 Dec 7;220(5171):976-9 PMID: 5701854
  35. Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes.
    Nature. 1967 Nov 18;216(5116):652-7 PMID: 6082459
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.5.1273
Published
1977-00-00
Pages
1273-89
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343755
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.5.1273 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2