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PMID: 3342018 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer.

The Biochemical journal ·Vol. 249 ·No. 1 ·1988-01-01 ·Pages 89-93

Comerford JG, Dawson AP

Abstract

1. GTP-promoted fusion between microsomal vesicles was studied by using fluorescence-resonance-energy transfer between the fluorescent membrane probes octadecanoyl-aminofluorescein and octadecyl-rhodamine. 2. The fluorescence increase after GTP addition does not require the presence of ATP, is unaffected by changes in free [Ca2+] in the range 10 microM-1 nM, but requires Mg2+, although higher Mg2+ concentrations are inhibitory. 3. In terms of requirements for poly(ethylene glycol), dependence on GTP concentration and inhibition by high Mg2+ concentrations, there is excellent correlation between rate of increase in fluorescence and rate of GTP-promoted Ca2+ efflux measured under Ca2+ transport conditions. 4. The observations support our previous conclusions that GTP-induced membrane fusion plays a major role in causing GTP-promoted Ca2+ efflux from microsomal vesicles.

MeSH Terms
Animals Biological Transport/drug effects Calcium/metabolism Guanosine Triphosphate/pharmacology Magnesium/pharmacology Male Microsomes, Liver/drug effects,metabolism Polyethylene Glycols/pharmacology Rats Spectrometry, Fluorescence
Chemicals
Polyethylene Glycols Guanosine Triphosphate Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Comerford J G
School of Biological Sciences, University of East Anglia, Norwich U.K.
Dawson A P
References (18)
18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2490089
Published
1988-01-01
Pages
89-93
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148670
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2490089 PMC Full Text View journal details
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