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PMID: 3499139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles.

The Biochemical journal ·Vol. 244 ·No. 1 ·1987-05-15 ·Pages 87-92

Dawson AP, Hills G, Comerford JG

Abstract

1. Guanosine 5'-[gamma-thio]triphosphate (GTP[S]), if added before GTP, blocks both Ca2+ efflux promoted by GTP and the effect of GTP on enhancement of inositol 1,4,5-triphosphate (IP3)-promoted Ca2+ release from preloaded microsomal vesicles. If, however, GTP[S] is added after GTP, it does not reverse the Ca2+ efflux promoted by GTP, nor does it inhibit IP3-promoted Ca2+ release. 2. The effect of GTP in enhancing IP3-promoted Ca2+ release is maintained after washing the microsomal vesicles free of added GTP. After this treatment, enhancement of IP3-promoted Ca2+ efflux can be observed in the absence of poly(ethylene glycol). 3. Electron microscopy shows that during GTP treatment of microsomal vesicles there is rapid production of very large vesicular structures, apparently produced by fusion of smaller vesicles. 4. Light-scattering changes are detectable during the fusion process. 5. Both Ca2+ efflux promoted by GTP and the enhancement of IP3-promoted Ca2+ release seen in the presence of GTP can probably be attributed to GTP-dependent vesicle fusion.

MeSH Terms
Animals Calcium/metabolism Guanosine Triphosphate/pharmacology Inositol 1,4,5-Trisphosphate Inositol Phosphates/pharmacology Light Male Microscopy, Electron Microsomes, Liver/drug effects,metabolism,ultrastructure Rats Scattering, Radiation
Chemicals
Inositol Phosphates Inositol 1,4,5-Trisphosphate Guanosine Triphosphate Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dawson A P
School of Biological Sciences, University of East Anglia, Norwich, U.K.
Hills G
Comerford J G
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2440087
Published
1987-05-15
Pages
87-92
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147957
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2440087 PMC Full Text View journal details
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