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PMID: 3356791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of gamma-hemolysin and lack of production of alpha-hemolysin by Staphylococcus aureus strains associated with toxic shock syndrome.

Journal of clinical microbiology ·Vol. 26 ·No. 3 ·1988-03-00 ·Pages 535-9

Clyne M, De Azavedo J, Carlson E, Arbuthnott J

Abstract

The hemolytic activity of toxic shock syndrome isolates of Staphylococcus aureus is enhanced when agarose is substituted for agar in blood plates or when strains are grown in liquid culture in the presence of 20% (vol/vol) CO2 in air. Hemolytic activity of a representative panel of toxic shock syndrome isolates was rigorously assessed both on blood agar and in liquid culture to unequivocally identify the predominant hemolysins produced. As determined by isoelectric focusing and Western immunoblotting, 15 of 15 TSS isolates produced gamma-lysin and 10 of 15 produced delta-lysin. None produced beta-lysin, and only 2 of 15 produced alpha-lysin. The low rate of alpha-lysin production was a most striking characteristic, since all strains were found to have the alpha-lysin gene by Southern blot hybridization.

MeSH Terms
Bacterial Proteins/biosynthesis Bacterial Toxins/biosynthesis,genetics Culture Media DNA, Bacterial/analysis Genes, Bacterial Hemolysin Proteins/biosynthesis,genetics Humans Immunoassay Isoelectric Focusing Nucleic Acid Hybridization Shock, Septic/microbiology Staphylococcus aureus/metabolism
Chemicals
Bacterial Proteins Bacterial Toxins Culture Media DNA, Bacterial Hemolysin Proteins gamma-hemolysin, Staphylococcus aureus staphylococcal alpha-toxin delta hemolysin protein, Staphylococcus aureus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clyne M
Department of Microbiology, Trinity College Dublin, Ireland.
De Azavedo J
Carlson E
Arbuthnott J
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19 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.26.3.535-539.1988
Published
1988-03-00
Pages
535-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC266327
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.26.3.535-539.1988 PMC Full Text View journal details
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