Mouse virulence of Salmonella typhimurium mutants deficient in two major outer membrane proteins.

Infection and immunity ·Vol. 18 ·No. 2 ·1977-11-00 ·Pages 454-8

Valtonen MV, Nurminen M, Johansson V, Makela PH

Abstract

The role of several outer membrane components as virulence factors is well established. We have now isolated spontaneous mutants and conjugational hybrids of smooth mouse virulent Salmonella typhimurium deficient in two major outer-membrane proteins. The lack of the 34,000- and/or 36,000-dalton proteins was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mutants and recombinants were then tested for their mouse virulence by intraperitoneal inoculation and were found to be as virulent as the parent strain.

MeSH Terms

Animals Bacterial Proteins/physiology Lethal Dose 50 Membrane Proteins/physiology Mice Mutation Recombination, Genetic Salmonella typhimurium/pathogenicity

Chemicals

Bacterial Proteins Membrane Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Valtonen M V

Nurminen M

Johansson V

Makela P H

References (15)

15 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.18.2.454-458.1977

Published

1977-11-00

Pages

454-8

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC421254

Subset

External Links

PubMed source DOI: 10.1128/iai.18.2.454-458.1977 PMC Full Text View journal details

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Mouse virulence of Salmonella typhimurium mutants deficient in two major outer membrane proteins.

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