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PMID: 337898 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.

Applied and environmental microbiology ·Vol. 34 ·No. 5 ·1977-11-00 ·Pages 518-22

Saunders GC, Bartlett ML

Abstract

A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.

MeSH Terms
Enterotoxins/analysis Food Contamination/analysis Immunoenzyme Techniques Staphylococcus
Chemicals
Enterotoxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Saunders G C
Bartlett M L
References (12)
12 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.34.5.518-522.1977
Published
1977-11-00
Pages
518-22
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC242693
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.34.5.518-522.1977 PMC Full Text View journal details
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