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PMID: 3380685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A quantitative analysis of nuclear factor I/DNA interactions.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4419-35

Meisterernst M, Gander I, Rogge L, Winnacker EL

Abstract

Nuclear factor I (NFI) was purified to homogeneity from porcine liver by DNA-affinity chromatography and displays a single band with a molecular weight of 36 kDa in SDS-polyacrylamide gels. The purified protein was used to determine absolute equilibrium binding constants by gel retardation techniques for a variety of DNA fragments with genuine or mutated NFI binding sites and a number of DNA fragments derived from various eukaryotic promoters carrying the CCAAT-box as a half-site for NFI binding. We present a model which allows prediction of the functional significance of mutated NFI binding-sites from sequence data. The data suggest that the single molecular species of NFI from porcine liver may not be able to recognize and activate the -CCAAT- promoter element in vivo without additional interactions, e.g. with other proteins.

MeSH Terms
Adenoviridae/genetics Adenoviruses, Human/genetics Animals Base Sequence CCAAT-Enhancer-Binding Proteins Chromatography, Affinity/methods DNA, Viral/genetics,metabolism DNA-Binding Proteins/isolation & purification,metabolism Kinetics Liver/metabolism Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Protein Binding Swine Transcription Factors Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Meisterernst M
Institut für Biochemie, Universität München, FRG.
Gander I
Rogge L
Winnacker E L
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.10.4419
Published
1988-05-25
Pages
4419-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336639
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.10.4419 PMC Full Text View journal details
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