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PMID: 3464831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial purification of a nuclear protein that binds to the CCAAT box of the mouse alpha 1-globin gene.

Molecular and cellular biology ·Vol. 6 ·No. 3 ·1986-03-00 ·Pages 821-32

Cohen RB, Sheffery M, Kim CG

Abstract

We enriched a fraction from nuclear extracts of murine erythroleukemia cells which contains a protein able to form stable complexes with the promoter region of the alpha 1-globin gene. Binding activity, which is present in mouse brain and a variety of cultured mouse and human cell lines, is not erythroid cell specific. Binding studies with alpha-globin gene promoter deletion mutants as well as DNase I footprinting and dimethyl sulfate protection studies showed that the factor bound specifically to the CCAAT box of the alpha 1 promoter. Enriched factor preparations exhibited weak binding to the promoter region of the beta maj-globin gene. This suggests that this protein could bind differentially to these two promoters in vivo. The enriched factor may be a ubiquitous nuclear protein involved in the differential regulation of the alpha 1- and beta maj-globin genes.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion DNA-Binding Proteins/isolation & purification,metabolism Genes Globins/genetics Leukemia, Erythroblastic, Acute Leukemia, Experimental Mice Mutation Nucleoproteins/isolation & purification,metabolism Promoter Regions, Genetic
Chemicals
DNA-Binding Proteins Nucleoproteins Globins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cohen R B
Sheffery M
Kim C G
References (54)
54 references, click to expand
  1. Eukaryotic gene transcription with purified components.
    Methods Enzymol. 1983;101:582-98 PMID: 6888276
  2. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  3. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 PMID: 6336326
  4. Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products.
    Cell. 1983 Nov;35(1):137-48 PMID: 6313220
  5. Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
    J Mol Biol. 1983 Nov 5;170(3):699-722 PMID: 6415291
  6. A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
    Cell. 1984 Feb;36(2):357-69 PMID: 6537904
  7. Gene expression in murine erythroleukemia cells. Transcriptional control and chromatin structure of the alpha 1-globin gene.
    J Mol Biol. 1984 Feb 5;172(4):417-36 PMID: 6319718
  8. Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 PMID: 6320177
  9. Molecular analysis of the envelope gene and long terminal repeat of Friend mink cell focus-inducing virus: implications for the functions of these sequences.
    J Virol. 1984 Mar;49(3):828-40 PMID: 6321768
  10. cis and trans activation of globin gene transcription in transient assays.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7428-32 PMID: 6200874
  11. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  12. A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
    Cell. 1984 Jul;37(3):889-901 PMID: 6540146
  13. Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences.
    Cell. 1984 Aug;38(1):251-63 PMID: 6205764
  14. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.
    Cell. 1984 Aug;38(1):265-73 PMID: 6088069
  15. Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin.
    Nature. 1984 Sep 6-11;311(5981):81-4 PMID: 6472466
  16. Is actin a transcription initiation factor for RNA polymerase B?
    EMBO J. 1984 Oct;3(10):2363-71 PMID: 6499833
  17. G to A substitution in the distal CCAAT box of the A gamma-globin gene in Greek hereditary persistence of fetal haemoglobin.
    Nature. 1985 Jan 24-30;313(6000):323-5 PMID: 2578619
  18. A point mutation in the A gamma-globin gene promoter in Greek hereditary persistence of fetal haemoglobin.
    Nature. 1985 Jan 24-30;313(6000):325-6 PMID: 2578620
  19. Specific protein binding to far upstream activating sequences in polymerase II promoters.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):43-7 PMID: 3881758
  20. Chromatin-specific hypersensitive sites are assembled on a Xenopus histone gene injected into Xenopus oocytes.
    J Mol Biol. 1985 Feb 5;181(3):333-49 PMID: 4039007
  21. A method for mapping intranuclear protein-DNA interactions and its application to a nuclease hypersensitive site.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2296-300 PMID: 3857580
  22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  23. Protein synthesis: its control in erythropoiesis.
    Science. 1972 Mar 3;175(4025):955-61 PMID: 5061867
  24. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  25. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  26. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  27. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  28. Recovery of DNA from gels.
    Methods Enzymol. 1980;65(1):371-80 PMID: 7374460
  29. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  30. E. coli RNA polymerase interacts homologously with two different promoters.
    Cell. 1980 Jun;20(2):269-81 PMID: 6248238
  31. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  32. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  33. The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.
    J Biol Chem. 1981 Feb 25;256(4):1960-7 PMID: 6161930
  34. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  35. Cloning and complete nucleotide sequence of human 5'-alpha-globin gene.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7054-8 PMID: 6452630
  36. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  37. Localization of DNA sequences necessary for transcription of the rabbit beta-globin gene in vitro.
    Cell. 1981 Jul;25(1):215-26 PMID: 7273136
  38. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  39. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
    Cell. 1981 Dec;27(2 Pt 1):279-88 PMID: 6277501
  40. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
    Cell. 1981 Dec;27(2 Pt 1):299-308 PMID: 6277502
  41. Murine erythroleukemia cell differentiation: DNase I hypersensitivity and DNA methylation near the globin genes.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1180-4 PMID: 6280172
  42. Rescuing the in vitro function of a globin pseudogene promoter.
    Nature. 1982 Jul 8;298(5870):192-5 PMID: 7088172
  43. Differences in human alpha-, beta- and delta-globin gene expression in monkey kidney cells.
    Cell. 1982 Aug;30(1):173-83 PMID: 6290076
  44. Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
    J Biol Chem. 1982 Dec 10;257(23):14419-27 PMID: 7142220
  45. Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure.
    Cell. 1982 Jul;29(3):887-93 PMID: 7151174
  46. The regulated expression of beta-globin genes introduced into mouse erythroleukemia cells.
    Cell. 1983 Feb;32(2):483-93 PMID: 6572107
  47. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  48. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  49. A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin.
    Cell. 1983 Apr;32(4):1191-203 PMID: 6301683
  50. Hexamethylenebisacetamide-resistant murine erythroleukemia cells have altered patterns of inducer-mediated chromatin changes.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3349-53 PMID: 6222378
  51. The nucleotide sequence of the rabbit embryonic globin gene beta 4.
    J Biol Chem. 1983 Jul 25;258(14):8739-44 PMID: 6305998
  52. Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
    Cell. 1985 May;41(1):21-30 PMID: 2986843
  53. Nucleosome disruption precedes transcription and is largely limited to the transcribed domain of globin genes in murine erythroleukemia cells.
    J Mol Biol. 1985 Mar 5;182(1):109-29 PMID: 2582137
  54. Regulated expression of the human beta-globin gene family in murine erythroleukaemia cells.
    Nature. 1983 Sep 22-28;305(5932):333-6 PMID: 6578416
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.3.821-832.1986
Published
1986-03-00
Pages
821-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367582
Subset
IM
Grants
NCI NIH HHS · CA-08748 · United States
NCI NIH HHS · CA-31768 · United States
Databases
GENBANK
M12931
External Links
PubMed source DOI: 10.1128/mcb.6.3.821-832.1986 PMC Full Text View journal details
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