Home LiteratureArticle Details
PMID: 3397182 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence analysis of the Streptococcus mutans scrB gene.

Infection and immunity ·Vol. 56 ·No. 8 ·1988-08-00 ·Pages 1956-60

Sato Y, Kuramitsu HK

Abstract

The complete nucleotide sequence of the Streptococcus mutans GS-5 scrB gene coding for sucrose-6-phosphate hydrolase activity was determined. A potential ribosome-binding site as well as promoter sequences were identified upstream from the gene. The deduced amino acid sequence of the enzyme suggested a molecular weight of 51,750, which is similar to that estimated for the enzyme isolated from strain GS-5. The enzyme is slightly acidic, with a pI of 5.9, and is a relatively hydrophilic protein. The nucleotide and amino acid sequences of the enzyme showed significant homology with those of the sacA protein from Bacillus subtilis. In addition, a region of amino acid homology with the S. mutans fructosyltransferase and B. subtilis levansucrase proteins was also detected.

MeSH Terms
Amino Acid Sequence Base Sequence Codon DNA, Bacterial/genetics Genes Genes, Bacterial Glycoside Hydrolases/genetics Molecular Sequence Data Sequence Homology, Nucleic Acid Streptococcus mutans/enzymology,genetics beta-Fructofuranosidase
Chemicals
Codon DNA, Bacterial Glycoside Hydrolases beta-Fructofuranosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sato Y
Department of Microbiology-Immunology, Northwestern University Medical-Dental Schools, Chicago, Illinois 60611.
Kuramitsu H K
References (25)
25 references, click to expand
  1. Localization of Streptococcus mutans GS-5 glucosyltransferases and intracellular invertase.
    Infect Immun. 1981 May;32(2):830-9 PMID: 6454662
  2. Initial characterization of sucrose-6-phosphate hydrolase from Streptococcus mutans and its apparent identity with intracellular invertase.
    Biochem Biophys Res Commun. 1979 Jul 12;89(1):307-14 PMID: 224874
  3. Sucrose transport by Streptococcus mutans. Evidence for multiple transport systems.
    Biochim Biophys Acta. 1982 Nov 22;692(3):415-24 PMID: 7171603
  4. Nucleotide sequence of the yeast SUC2 gene for invertase.
    Nucleic Acids Res. 1983 Mar 25;11(6):1943-54 PMID: 6300785
  5. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites.
    Mol Gen Genet. 1985;200(2):220-8 PMID: 2993818
  6. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.
    J Bacteriol. 1985 Nov;164(2):782-96 PMID: 2997130
  7. Complete nucleotide sequence of type 6 M protein of the group A Streptococcus. Repetitive structure and membrane anchor.
    J Biol Chem. 1986 Feb 5;261(4):1677-86 PMID: 3511046
  8. Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase.
    J Bacteriol. 1986 May;166(2):426-34 PMID: 3009399
  9. Isolation and characterization of the sucrose 6-phosphate hydrolase gene from Streptococcus mutans.
    Infect Immun. 1986 Sep;53(3):582-6 PMID: 3017864
  10. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis.
    Infect Immun. 1986 Sep;53(3):587-94 PMID: 3017865
  11. Gene for an immunoglobulin-binding protein from a group G streptococcus.
    J Bacteriol. 1986 Sep;167(3):870-80 PMID: 3745123
  12. Role of Streptococcus mutans in human dental decay.
    Microbiol Rev. 1986 Dec;50(4):353-80 PMID: 3540569
  13. Nucleotide sequence of the sucrase gene of Bacillus subtilis.
    Gene. 1986;45(2):221-5 PMID: 3100393
  14. Sequence analysis of the gtfB gene from Streptococcus mutans.
    J Bacteriol. 1987 Sep;169(9):4263-70 PMID: 3040685
  15. Identification of the genes for the lactose-specific components of the phosphotransferase system in the lac operon of Staphylococcus aureus.
    J Biol Chem. 1987 Dec 5;262(34):16444-9 PMID: 2824493
  16. Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions.
    J Bacteriol. 1988 Feb;170(2):810-6 PMID: 2828325
  17. Utilization of a mini-mu transposon to construct defined mutants in Streptococcus mutans.
    Mol Microbiol. 1987 Sep;1(2):229-32 PMID: 2835581
  18. Sucrose metabolism by Streptococcus mutans, SL-I.
    Biochim Biophys Acta. 1971 Feb 28;261(2):379-87 PMID: 4111389
  19. Characterization of invertase activity from cariogenic Streptococcus mutans.
    J Bacteriol. 1973 Sep;115(3):1003-10 PMID: 4353868
  20. Identification, preliminary characterization, and evidence for regulation of invertase in Streptococcus mutans.
    J Bacteriol. 1973 Oct;116(1):192-202 PMID: 4745413
  21. Four types of Streptococcus mutans based on their genetic, antigenic and biochemical characteristics.
    J Gen Microbiol. 1974 Aug;83(2):327-38 PMID: 4427102
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Purification and antigenic properties of intracellular invertase from Streptococcus mutans.
    Infect Immun. 1979 Mar;23(3):873-83 PMID: 457262
  24. Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans.
    Infect Immun. 1979 Jun;24(3):865-8 PMID: 468378
  25. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.8.1956-1960.1988
Published
1988-08-00
Pages
1956-60
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259507
Subset
IM
Grants
NIDCR NIH HHS · DE-03258 · United States
External Links
PubMed source DOI: 10.1128/iai.56.8.1956-1960.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2