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PMID: 340905 Published · ppublish English Journal Article

A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.

Molecular & general genetics : MGG ·Vol. 156 ·No. 3 ·1977-11-18 ·Pages 233-7

Isaksson LA, Sköld SE, Skjöldebrand J, Takata R

Abstract

A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA or protein or both at restrictive temperature.

MeSH Terms
Bacterial Proteins/biosynthesis Escherichia coli/genetics,isolation & purification Genetic Techniques Hot Temperature Mutation RNA, Bacterial/biosynthesis
Chemicals
Bacterial Proteins RNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Isaksson L A
Sköld S E
Skjöldebrand J
Takata R
References (27)
27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267177
Published
1977-11-18
Pages
233-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267177
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