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PMID: 341155 Published · ppublish English Journal Article

mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5387-91

Chu F, Caldwell P, Weissbach H, Brot N

Abstract

RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be bound to L12-depleted ribosomes and the synthesized elongation factor Tu can form complexes with both elongation factor Ts and GDP. Guanosine 5'-diphosphate 3'-diphosphate has no effect on the synthesis of these proteins from an RNA template but inhibits their synthesis when a DNA template is used.

MeSH Terms
Cell-Free System Escherichia coli/metabolism Guanosine Diphosphate/metabolism Guanosine Tetraphosphate/pharmacology Immunoassay Peptide Elongation Factors RNA, Messenger/metabolism Ribosomal Proteins/biosynthesis,immunology Ribosomes/metabolism
Chemicals
Peptide Elongation Factors RNA, Messenger Ribosomal Proteins Guanosine Diphosphate Guanosine Tetraphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chu F
Caldwell P
Weissbach H
Brot N
References (47)
47 references, click to expand
  1. DNA-directed in vitro synthesis of elongation factor Tu.
    Biochem Biophys Res Commun. 1976 Dec 20;73(4):917-27 PMID: 15625861
  2. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1964 Dec;52:1367-74 PMID: 14243510
  3. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  4. DNA-dependent in vitro synthesis of fibosomal proteins, protein elongation factors, and RNA polymerase subunit alpha: inhibition by ppGpp.
    Cell. 1976 Nov;9(3):439-48 PMID: 991274
  5. Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templates.
    J Mol Biol. 1977 Feb 15;110(1):75-87 PMID: 845948
  6. DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 and the formation of an L10L12 complex.
    Biochem Biophys Res Commun. 1976 May 23;76(2):593-601 PMID: 800342
  7. Accumulation of free ribosomal proteins S1, L7, and L12 in Escherichia coli.
    Eur J Biochem. 1976 Oct 1;69(1):289-97 PMID: 791647
  8. DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3156-9 PMID: 787986
  9. Stringent control of ribosomal protein gene expression in Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):3819-23 PMID: 4610562
  10. In vitro synthesis of ribosomal proteins directed by Escherichia coli DNA.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):446-50 PMID: 4205593
  11. Requirement of an Escherichia coli 50 S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate.
    J Biol Chem. 1972 Feb 10;247(3):805-14 PMID: 4333515
  12. Accumulation and turnover of guanosine tetraphosphate in Escherichia coli.
    J Mol Biol. 1972 Nov 28;71(3):769-83 PMID: 4567473
  13. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.
    J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
  14. Further studies on the role of 50 S ribosomal proteins in protein synthesis.
    J Biol Chem. 1973 Oct 25;248(20):6952-6 PMID: 4582729
  15. RNA-directed cell-free synthesis of the galactose enzymes of Escherichia coli.
    Mol Gen Genet. 1973 Aug 28;124(4):329-44 PMID: 4355744
  16. Studies on the role of ribosomal proteins L 7 and L 12 in the in vitro synthesis of -galactosidase.
    J Biol Chem. 1973 Jul 25;248(14):5012-5 PMID: 4352186
  17. Structural and functional evidence for a repeated 50S subunit ribosomal protein.
    Nat New Biol. 1973 Mar 14;242(115):47-9 PMID: 4571811
  18. DNA-dependent in vitro synthesis of enzymes of the galactose operon of Escherichia coli.
    Mol Gen Genet. 1971;112(1):14-27 PMID: 4330068
  19. The messenger-directed synthesis of the alpha-fragment of the enzyme beta-galactosidase.
    Biochem Biophys Res Commun. 1973 Aug 6;53(3):967-74 PMID: 4354455
  20. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743-7 PMID: 1101264
  21. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.
    Nature. 1975 Oct 9;257(5526):458-62 PMID: 1101072
  22. Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.
    J Biol Chem. 1975 Feb 25;250(4):1556-62 PMID: 1089661
  23. Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4149-53 PMID: 4610570
  24. Separation and radioautography of microgram quantities of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis.
    FEBS Lett. 1973 Jan 15;29(2):177-80 PMID: 4578220
  25. The mechanism of action of ppGpp on rRNA synthesis in vitro.
    Cell. 1976 May;8(1):123-8 PMID: 8211
  26. 50-S ribosomal proteins. Purification and partial characterization of two acidic proteins, A 1 and A 2, isolated from 50-S ribosomes of Escherichia coli.
    Eur J Biochem. 1972 Jan 31;25(1):5-12 PMID: 4554105
  27. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  28. DNA-dependent in vitro synthesis of bacteriophage enzymes.
    Cold Spring Harb Symp Quant Biol. 1969;34:763-6 PMID: 4985891
  29. Cell-free synthesis of tryptophanase from Escherichia coli. Use of ribonucleic acid isolated from induced cells and a comparison of the product from a system employing ribosomes with that from one employing ribosomes and exogenous ribonucleic acid.
    Biochem J. 1971 Nov;125(2):643-53 PMID: 4947396
  30. Purification of factor Ts: studies on the formation and stability of nucleotide complexes containing transfer factor Tu.
    Arch Biochem Biophys. 1971 Dec;147(2):457-66 PMID: 4944516
  31. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  32. Studies on the purification and properties of factor Tu from E. coli.
    Arch Biochem Biophys. 1970 Nov;141(1):26-37 PMID: 4921066
  33. An interaction between the transfer factors required for protein synthesis.
    Arch Biochem Biophys. 1969 Jun;132(1):146-50 PMID: 4893410
  34. In vitro synthesis of Escherichia coli alkaline phosphatase monomers.
    J Mol Biol. 1971 Jun 14;58(2):469-79 PMID: 4933414
  35. Translational control of protein synthesis.
    Annu Rev Biochem. 1976;45:39-72 PMID: 786155
  36. The identification and characterization of proteins similar to L7, L12 in ribosome-free extracts of Escherichia coli.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):293-302 PMID: 807213
  37. Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome.
    J Mol Biol. 1975 Jun 15;95(1):1-8 PMID: 1097708
  38. Stringent control of the transcriptional activities of ribosomal protein genes in E. coli.
    Nature. 1975 Jun 5;255(5508):460-5 PMID: 1094295
  39. Pools of ribosomal proteins in Escherichia coli. Studies on the exchange of proteins between pools and ribosomes.
    Eur J Biochem. 1975 Sep 1;57(1):49-54 PMID: 1100403
  40. Effects of guanosine tetraphosphate on cell-free synthesis of Escherichia coli ribosomal RNA and other gene products.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2881-5 PMID: 1103124
  41. Syntheses of elongation factors Tu and G are under stringent control in Escherichia coli.
    J Biol Chem. 1976 Feb 10;251(3):898-901 PMID: 765336
  42. Content of elongation factor Tu in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4780-4 PMID: 1108000
  43. The stoichiometry of the ribosomal proteins of Escherichia coli.
    Mol Gen Genet. 1975 Oct 3;140(3):253-74 PMID: 1107798
  44. The ribosomal protein L8 is a complex L7/L12 and L10.
    FEBS Lett. 1976 Apr 15;64(1):135-8 PMID: 773698
  45. Radioactive labeling of proteins in vitro.
    J Biol Chem. 1971 Feb 10;246(3):831-2 PMID: 5100767
  46. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  47. In vitro synthesis of bacteriophage lysozyme.
    Nature. 1967 Aug 5;215(5101):588-91 PMID: 6050209
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5387
Published
1977-12-00
Pages
5387-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431730
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5387 PMC Full Text View journal details
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