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PMID: 3416832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Four classes of mRNA are expressed from the mouse int-2 gene, a member of the FGF gene family.

The EMBO journal ·Vol. 7 ·No. 7 ·1988-07-00 ·Pages 2035-41

Mansour SL, Martin GR

Abstract

Mouse embryos at 7.5 days of gestation and endodermal cells derived from embryonal carcinoma cells each express four int-2 mRNAs of similar size and relative abundance. To determine their structure and coding potential, we prepared a cDNA library from endoderm mRNA and isolated several int-2 cDNAs. Structural analysis of these cDNAs combined with Northern blot hybridization and primer extension analyses of int-2 mRNA revealed that the differences between the mRNAs are generated through the use of two alternate transcriptional start sites and two alternate polyadenylation sites. All four mRNAs share a common core sequence that encodes a protein with amino acid similarity to fibroblast growth factor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA/genetics Endoderm/metabolism Fibroblast Growth Factors/genetics Genes Mice Molecular Sequence Data Nucleic Acid Hybridization RNA, Messenger/genetics Teratoma Transcription, Genetic
Chemicals
RNA, Messenger Fibroblast Growth Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mansour S L
Department of Anatomy, University of California, San Francisco 94143.
Martin G R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-07-00
Pages
2035-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454479
Subset
IM
Grants
NCI NIH HHS · 2T32 CA09043 · United States
External Links
PubMed source PMC Full Text View journal details
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