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PMID: 3474496 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Inc3B determinant of plasmid pT181. A mutational analysis.

Molecular & general genetics : MGG ·Vol. 207 ·No. 1 ·1987-04-00 ·Pages 60-7

Iordanescu S

Abstract

A region encompassing the origin of replication of staphylococcal plasmid pT181 has previously been shown to express an incompatibility effect denoted Inc3B, when cloned into another replicon (Novick et al. 1984). In an attempt to understand the mechanism of this incompatibility effect, and its relationship with the function of the replication origin, mutants deficient in this property were isolated and characterized. The results obtained suggest that the Inc3B effect is due to the competition for replication between the replication origin cloned in a hybrid and the origin of an autonomous plasmid. The Inc3B-deficient mutants isolated expressed different degrees of residual incompatibility. The inc3B mutations which did not express any incompatibility were found also to inactivate the function of the replication origin. All the other mutants which expressed residual Inc3B had a functional origin but presented a significantly reduced ability to use this origin when coexisting with a plasmid using a wild-type pT181 origin. It is suggested that these inc3B mutations represent a new type of origin mutation which affects the ability of the origin to compete with other origins using the same replication system, though the function per se of the origin is not significantly impaired.

MeSH Terms
DNA Replication DNA, Bacterial/biosynthesis Genetic Vectors Mutation Phenotype Plasmids Staphylococcus aureus/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Iordanescu S
References (37)
37 references, click to expand
  1. Interaction of the plasmid R6K-encoded replication initiator protein with its binding sites on DNA.
    Cell. 1983 Aug;34(1):125-34 PMID: 6224568
  2. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps.
    Plasmid. 1979 Jan;2(1):109-29 PMID: 314115
  3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  4. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4108-12 PMID: 6287465
  5. Structure of replication origin of the Escherichia coli K-12 chromosome: the presence of spacer sequences in the ori region carrying information for autonomous replication.
    Nucleic Acids Res. 1982 Jun 25;10(12):3745-54 PMID: 6287420
  6. Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1150-4 PMID: 375227
  7. Genetic studies of F plasmid maintenance genes involved in copy number control, incompatability, and partitioning.
    Plasmid. 1982 Mar;7(2):163-79 PMID: 6281833
  8. Plasmid R6K DNA replication. II. Direct nucleotide sequence repeats are required for an active gamma-origin.
    J Mol Biol. 1982 Oct 15;161(1):45-56 PMID: 6296394
  9. The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19.
    Mol Gen Genet. 1979 Mar 27;171(3):277-85 PMID: 377014
  10. Three distinct plasmids originating in the same Staphylococcus aureus strain.
    Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111-8 PMID: 1008685
  11. Isolation and complementation of temperature-sensitive replication mutants of Staphylococcus aureus plasmid pC194.
    Mol Gen Genet. 1983;191(2):201-6 PMID: 6578397
  12. Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6814-8 PMID: 6316326
  13. Removal of symmetrical sequences from the origin region of plasmid R1 reduces its replication efficiency.
    EMBO J. 1983;2(5):657-62 PMID: 16453449
  14. Plasmid pT181 replication is regulated by two countertranscripts.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):638-42 PMID: 2579377
  15. Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.
    J Bacteriol. 1980 Jan;141(1):111-20 PMID: 6986353
  16. Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance.
    J Bacteriol. 1980 Jan;141(1):213-22 PMID: 6243621
  17. Plasmid DNA replication: RK2- and R6K-encoded trans-acting factors and their sites of action.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:91-7 PMID: 383388
  18. Plasmid transfer and genetic recombination by protoplast fusion in staphylococci.
    J Bacteriol. 1981 Jan;145(1):74-81 PMID: 7007333
  19. Copy mutant of mini-Rts1: lowered binding affinity of mutated RepA protein to direct repeats.
    J Bacteriol. 1985 Apr;162(1):72-7 PMID: 3156849
  20. Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein.
    EMBO J. 1984 Oct;3(10):2399-405 PMID: 6499834
  21. Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2.
    Mol Gen Genet. 1981;181(1):8-12 PMID: 6261086
  22. Effect of the deletion of a fragment dispensable for the autonomous maintenance of plasmid pT181 on the competition between incompatible plasmids.
    Plasmid. 1986 May;15(3):191-8 PMID: 3520616
  23. Sequence organization of replication origin of the Escherichia coli K-12 chromosome.
    J Mol Biol. 1984 Jul 15;176(4):443-58 PMID: 6379192
  24. Control of pT181 replication II. Mutational analysis.
    EMBO J. 1984 Oct;3(10):2407-14 PMID: 6437809
  25. Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus.
    Mol Gen Genet. 1985;199(3):452-64 PMID: 2993795
  26. Transduction-related cointegrate formation between Staphylococcal plasmids: a new type of site-specific recombination.
    Plasmid. 1981 Sep;6(2):159-72 PMID: 6458059
  27. Plasmid P1 replication: negative control by repeated DNA sequences.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6456-60 PMID: 6387706
  28. Incompatibility of plasmids containing the replication origin of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5347-51 PMID: 6291040
  29. Staphylococcus aureus chromosomal mutation specifically affecting the copy number of Inc3 plasmids.
    Plasmid. 1983 Sep;10(2):130-7 PMID: 6635012
  30. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.
    J Bacteriol. 1975 Nov;124(2):597-601 PMID: 1184573
  31. Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.
    J Bacteriol. 1983 Jul;155(1):337-44 PMID: 6305917
  32. Complementation of a plasmid replication defect by autonomous incompatible plasmids in Staphylococcus aureus.
    Plasmid. 1980 Jul;4(1):1-7 PMID: 6927766
  33. cmp, a cis-acting plasmid locus that increases interaction between replication origin and initiator protein.
    J Bacteriol. 1986 Oct;168(1):160-6 PMID: 3759903
  34. Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.
    Mol Gen Genet. 1982;186(3):372-7 PMID: 6750320
  35. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus.
    Genetics. 1974 Apr;76(4):681-702 PMID: 4275652
  36. Functional origin of replication of pT181 plasmid DNA is contained within a 168-base-pair segment.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4580-4 PMID: 6956881
  37. Replication control system in lambda dv.
    Plasmid. 1981 Jan;5(1):32-52 PMID: 6452646
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331491
Published
1987-04-00
Pages
60-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331491
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