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PMID: 3486420 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a cDNA coding for human factor VII.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2412-6

Hagen FS, Gray CL, O'Hara P, Grant FJ, Saari GC, Woodbury RG, Hart CE, Insley M, Kisiel W, Kurachi K

Abstract

Factor VII is a precursor to a serine protease that is present in mammalian plasma. In its activated form, it participates in blood coagulation by activating factor X and/or factor IX in the presence of tissue factor and calcium. Clones coding for factor VII were obtained from two cDNA libraries prepared from poly(A) RNA from human liver and Hep G2 cells. The amino acid sequence deduced from the cDNAs indicates that factor VII is synthesized with a prepro-leader sequence of 60 or 38 amino acids. The mature protein that circulates in plasma is a single-chain polypeptide composed of 406 amino acids. The amino acid sequence analysis of the protein and the amino acid sequence deduced from the cDNAs indicate that factor VII is converted to factor VIIa by the cleavage of a single internal bond between arginine and isoleucine. This results in the formation of a light chain (152 amino acids) and a heavy chain (254 amino acids) that are held together by a disulfide bond. The light chain contains a gamma-carboxyglutamic acid (Gla) domain and two potential epidermal growth factor domains, while the heavy chain contains the serine protease portion of the molecule. Factor VII shows a high degree of amino acid sequence homology with the other vitamin K-dependent plasma proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Calcium-Binding Proteins/genetics Cloning, Molecular DNA/genetics Factor VII/genetics Gene Expression Regulation Genetic Vectors Growth Substances/genetics Humans Osteocalcin Protein Sorting Signals/genetics
Chemicals
Calcium-Binding Proteins Growth Substances Protein Sorting Signals Osteocalcin Factor VII DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hagen F S
Gray C L
O'Hara P
Grant F J
Saari G C
Woodbury R G
Hart C E
Insley M
Kisiel W
Kurachi K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2412
Published
1986-04-00
Pages
2412-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323307
Subset
IM
Grants
NHLBI NIH HHS · HL 16919 · United States
Databases
GENBANK
M13232
External Links
PubMed source DOI: 10.1073/pnas.83.8.2412 PMC Full Text View journal details
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