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PMID: 3522554 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of leader peptide synthesis in tryptophanase operon expression in Escherichia coli K-12.

Journal of bacteriology ·Vol. 167 ·No. 1 ·1986-07-00 ·Pages 383-6

Stewart V, Yanofsky C

Abstract

We used site-directed mutagenesis to replace the Escherichia coli tryptophanase (tna) operon leader peptide start codon with AUC. This change greatly decreased the uninduced rate of tna operon expression, and it also lowered the response to inducer. We conclude that leader peptide synthesis plays an essential role in tna operon expression.

MeSH Terms
Escherichia coli/enzymology,genetics Gene Expression Regulation Lyases/genetics Mutation Operon Protein Sorting Signals/biosynthesis Transcription, Genetic Tryptophan/pharmacology Tryptophanase/biosynthesis,genetics
Chemicals
Protein Sorting Signals Tryptophan Lyases Tryptophanase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stewart V
Yanofsky C
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19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.1.383-386.1986
Published
1986-07-00
Pages
383-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212888
Subset
IM
Grants
NIGMS NIH HHS · GM08736 · United States
NIGMS NIH HHS · GM09738 · United States
External Links
PubMed source DOI: 10.1128/jb.167.1.383-386.1986 PMC Full Text View journal details
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