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PMID: 3525516 Published · ppublish English Journal Article

Molecular analysis of the promoter operator region of the Escherichia coli K-12 tyrP gene.

Journal of bacteriology ·Vol. 167 ·No. 2 ·1986-08-00 ·Pages 556-61

Kasian PA, Davidson BE, Pittard J

Abstract

The nucleotide sequence of the tyrP promoter region from Escherichia coli has been determined. Two TYR R boxes have been identified, and one of these was shown to overlap the -35 region of a major tyrP promoter (p1). S1 nuclease mapping of in vivo transcripts revealed that transcription from p1 is stimulated by phenylalanine and to a lesser extent by leucine. The demonstration that mutants in which TyrR-tyrosine-mediated repression of tyrP has been abolished have single base changes in the TYR R box which overlaps p1 suggests that TyrR-tyrosine-mediated repression of tyrP also involves p1. TyrR-independent stimulation of tyrP expression by Casamino Acids involves a second promoter 140 bases upstream of p1. There are no TYR R boxes in this region. The sequences of 10 TYR R boxes preceding the genes tyrP, tyrR, and aroG and the operons aroF tyrA and aroL aroM are compared and discussed.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Chromosome Mapping Escherichia coli/genetics Genes, Bacterial Operator Regions, Genetic Plasmids Promoter Regions, Genetic Repressor Proteins/genetics Transcription, Genetic Tyrosine/genetics,metabolism
Chemicals
Bacterial Proteins Repressor Proteins Tyrosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kasian P A
Davidson B E
Pittard J
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.2.556-561.1986
Published
1986-08-00
Pages
556-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212925
Subset
IM
Databases
GENBANK
M13768
External Links
PubMed source DOI: 10.1128/jb.167.2.556-561.1986 PMC Full Text View journal details
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