Home LiteratureArticle Details
PMID: 3539942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alteration of the cytoplasmic domain of the membrane-spanning glycoprotein p62 of Semliki Forest virus does not affect its polar distribution in established lines of Madin-Darby canine kidney cells.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 2 ·1986-12-00 ·Pages 2607-18

Roman LM, Garoff H

Abstract

Expression of the Semliki Forest virus p62/E2 protein was studied in the polarized epithelial cell line Madin-Darby canine kidney (MDCK). After infection this transmembrane protein, together with the other spike subunit E1, accumulates at the basolateral surface of MDCK cells (Fuller, S. D., C.-H. von Bonsdorff, and K. Simons, 1985, EMBO (Eur. Mol. Biol. Organ.) J., 4:2475-2485). The cDNAs encoding truncated forms of the protein were used to stably transform MDCK cells to examine the role of subunit oligomerization (E1-E2) and the cytoplasmic domain of p62/E2 in directed transport to the basolateral surface. The biochemical characteristics and polarity of the expressed proteins were studied using cell monolayers grown on nitrocellulose filters. A wild-type form of p62/E2, in the absence of E1, and two forms having either 15 or 3 of the wild-type 31-amino acid carboxyl cytoplasmic domain were all localized to the basolateral surface. These results indicate that the cytoplasmic domain of E2 does not contain the information essential for directed transport to the plasma membrane, and imply that this information resides in either the lumenal and/or membrane-spanning segments of this transmembrane protein.

MeSH Terms
Animals Cell Compartmentation Cell Line Cell Membrane/metabolism,ultrastructure Cloning, Molecular Cytoplasm Dogs Epithelium/metabolism,ultrastructure Fatty Acids/metabolism Fluorescent Antibody Technique Glycoproteins/genetics,metabolism Glycosylation Macromolecular Substances Membrane Proteins/genetics,metabolism Protein Processing, Post-Translational Semliki forest virus/genetics Structure-Activity Relationship Viral Proteins/genetics,metabolism
Chemicals
Fatty Acids Glycoproteins Macromolecular Substances Membrane Proteins Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roman L M
Garoff H
References (65)
65 references, click to expand
  1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  2. Studies in subviral components of Semliki Forest virus.
    Ann Med Exp Biol Fenn. 1969;47(4):235-48 PMID: 5393678
  3. Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.
    J Biol Chem. 1974 Feb 10;249(3):811-7 PMID: 4204552
  4. Selective solubilization of proteins from red blood cell membranes by protein perturbants.
    J Supramol Struct. 1973;1(3):220-32 PMID: 4804837
  5. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  6. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
    J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
  7. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  8. Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5584-8 PMID: 146200
  9. Polarized monolayers formed by epithelial cells on a permeable and translucent support.
    J Cell Biol. 1978 Jun;77(3):853-80 PMID: 567227
  10. Assembly of the Semliki Forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro.
    J Mol Biol. 1978 Oct 5;124(4):587-600 PMID: 712848
  11. Evidence for an autoprotease activity of sindbis virus capsid protein.
    Virology. 1978 Oct 15;90(2):366-9 PMID: 726255
  12. Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5071-5 PMID: 283416
  13. Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
    J Cell Biol. 1979 Jun;81(3):635-48 PMID: 222773
  14. Polarity of influenza and vesicular stomatitis virus maturation in MDCK cells: lack of a requirement for glycosylation of viral glycoproteins.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6430-4 PMID: 230510
  15. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  16. Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum.
    J Cell Biol. 1980 NOV;87(2 Pt 1):503-8 PMID: 7430254
  17. Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4132-6 PMID: 6933462
  18. Formation of the Semliki Forest virus membrane glycoprotein complexes in the infected cell.
    J Gen Virol. 1980 Sep;50(1):111-23 PMID: 7441208
  19. Phase separation of integral membrane proteins in Triton X-114 solution.
    J Biol Chem. 1981 Feb 25;256(4):1604-7 PMID: 6257680
  20. Identification of two strains of MDCK cells which resemble separate nephron tubule segments.
    Biochim Biophys Acta. 1981 Feb 18;673(1):26-36 PMID: 6110442
  21. Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7112-6 PMID: 6938958
  22. Glycosylation does not determine segregation of viral envelope proteins in the plasma membrane of epithelial cells.
    J Cell Biol. 1981 May;89(2):230-9 PMID: 6265461
  23. Transport properties of toad kidney epithelia in culture.
    Am J Physiol. 1981 Sep;241(3):C154-9 PMID: 6269434
  24. Influence of collagen gel on the orientation of epithelial cell polarity: follicle formation from isolated thyroid cells and from preformed monolayers.
    J Cell Biol. 1981 Oct;91(1):157-66 PMID: 7298715
  25. Mechanisms for the incorporation of proteins in membranes and organelles.
    J Cell Biol. 1982 Jan;92(1):1-22 PMID: 7035466
  26. Antibodies to the Golgi complex and the rough endoplasmic reticulum.
    J Cell Biol. 1982 Jan;92(1):92-107 PMID: 7199056
  27. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.
    J Cell Biol. 1982 Apr;93(1):97-102 PMID: 7068762
  28. Structure and assembly of alphaviruses.
    Curr Top Microbiol Immunol. 1982;99:1-50 PMID: 6284443
  29. Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells.
    Cell. 1982 Oct;30(3):753-62 PMID: 6291783
  30. Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein.
    Nature. 1982 Dec 16;300(5893):598-603 PMID: 7144911
  31. Proteins IEF (isoelectric focusing) 31 and IEF 46 are keratin-type components of the intermediate-sized filaments: keratins of various human cultured epithelial cells.
    J Cell Biol. 1983 Feb;96(2):416-23 PMID: 6187751
  32. A domain-specific marker for the hepatocyte plasma membrane: localization of leucine aminopeptidase to the bile canalicular domain.
    J Cell Biol. 1983 Jun;96(6):1548-58 PMID: 6304108
  33. Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA.
    Cell. 1983 Jun;33(2):435-43 PMID: 6305510
  34. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  35. Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.
    Cell. 1983 Aug;34(1):233-43 PMID: 6883510
  36. Expression of Semliki Forest virus proteins from cloned complementary DNA. I. The fusion activity of the spike glycoprotein.
    J Cell Biol. 1983 Sep;97(3):644-51 PMID: 6688423
  37. Expression of Semliki Forest virus proteins from cloned complementary DNA. II. The membrane-spanning glycoprotein E2 is transported to the cell surface without its normal cytoplasmic domain.
    J Cell Biol. 1983 Sep;97(3):652-8 PMID: 6688424
  38. Altered cytoplasmic domains affect intracellular transport of the vesicular stomatitis virus glycoprotein.
    Cell. 1983 Sep;34(2):513-24 PMID: 6352053
  39. Expression and function of transplantation antigens with altered or deleted cytoplasmic domains.
    Cell. 1983 Sep;34(2):535-44 PMID: 6604582
  40. Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1381-8 PMID: 6415065
  41. Spectrophotometric quantitation of silver grains eluted from autoradiograms.
    Anal Biochem. 1983 Sep;133(2):511-4 PMID: 6638510
  42. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation.
    Cell. 1983 Dec;35(2 Pt 1):531-8 PMID: 6317196
  43. A domain-specific marker for the hepatocyte plasma membrane. III. Isolation of bile canalicular membrane by immunoadsorption.
    J Cell Biol. 1984 Apr;98(4):1497-504 PMID: 6371022
  44. Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK.
    Cell. 1984 Aug;38(1):65-77 PMID: 6088077
  45. Semliki Forest virus: a probe for membrane traffic in the animal cell.
    Adv Protein Chem. 1984;36:79-132 PMID: 6382965
  46. Biogenesis of epithelial cell polarity: intracellular sorting and vectorial exocytosis of an apical plasma membrane glycoprotein.
    Cell. 1984 Dec;39(3 Pt 2):537-46 PMID: 6509551
  47. Development of cell surface polarity in the epithelial Madin-Darby canine kidney (MDCK) cell line.
    EMBO J. 1984 Nov;3(11):2687-94 PMID: 6391916
  48. Polarized delivery of viral glycoproteins to the apical and basolateral plasma membranes of Madin-Darby canine kidney cells infected with temperature-sensitive viruses.
    J Cell Biol. 1985 Jan;100(1):136-51 PMID: 2981229
  49. Mutations in the cytoplasmic domain of the influenza virus hemagglutinin affect different stages of intracellular transport.
    J Cell Biol. 1985 Mar;100(3):704-14 PMID: 3972890
  50. An artificial anchor domain: hydrophobicity suffices to stop transfer.
    Cell. 1985 Jun;41(2):607-14 PMID: 3886166
  51. Internalization-defective LDL receptors produced by genes with nonsense and frameshift mutations that truncate the cytoplasmic domain.
    Cell. 1985 Jul;41(3):735-43 PMID: 3924410
  52. Protein fatty acyltransferase is located in the rough endoplasmic reticulum.
    FEBS Lett. 1985 Aug 5;187(2):289-94 PMID: 4018267
  53. Intracellular sorting and basolateral appearance of the G protein of vesicular stomatitis virus in Madin-Darby canine kidney cells.
    J Cell Biol. 1985 Aug;101(2):470-6 PMID: 2991300
  54. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer.
    J Cell Biol. 1985 Aug;101(2):639-45 PMID: 2991303
  55. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  56. Analysis of the differentiation-promoting potential of inducible c-fos genes introduced into embryonal carcinoma cells.
    EMBO J. 1985 Jul;4(7):1775-81 PMID: 3896777
  57. Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
    EMBO J. 1985 Oct;4(10):2475-85 PMID: 2996880
  58. Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein.
    J Cell Biol. 1985 Dec;101(6):2199-209 PMID: 2999159
  59. Exocytotic pathways exist to both the apical and the basolateral cell surface of the polarized epithelial cell MDCK.
    Cell. 1985 Nov;43(1):297-306 PMID: 4075397
  60. Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail.
    J Cell Biol. 1986 Jan;102(1):1-10 PMID: 3941149
  61. Mutants of the membrane-binding region of Semliki Forest virus E2 protein. I. Cell surface transport and fusogenic activity.
    J Cell Biol. 1986 Mar;102(3):889-901 PMID: 3753980
  62. Mutants of the membrane-binding region of Semliki Forest virus E2 protein. II. Topology and membrane binding.
    J Cell Biol. 1986 Mar;102(3):902-10 PMID: 3949882
  63. Surface expression of viral glycoproteins is polarized in epithelial cells infected with recombinant vaccinia viral vectors.
    EMBO J. 1986 Feb;5(2):237-45 PMID: 3011398
  64. Cell surface polarity in epithelia.
    Annu Rev Cell Biol. 1985;1:243-88 PMID: 3939606
  65. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2607
Published
1986-12-00
Pages
2607-18
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114582
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.103.6.2607 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2