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PMID: 3542835 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparative study of Vibrio cholerae non-O1 protease and soluble hemagglutinin with those of Vibrio cholerae O1.

Infection and immunity ·Vol. 55 ·No. 2 ·1987-02-00 ·Pages 451-4

Honda T, Booth BA, Boesman-Finkelstein M, Finkelstein RA

Abstract

Protease and soluble hemagglutinating activities produced by a non-O1 Vibrio cholerae strain isolated from a patient with diarrhea were compared with similar activities produced by V. cholerae O1. The soluble protease activities were indistinguishable in heat stability, immunodiffusion, inhibition by antiserum, and electrophoretic analysis. On the other hand, the soluble hemagglutinating activities of both strains were not completely identical. The hemagglutinating activity of the non-O1 V. cholerae strain was not inhibited by Zincov; it was more sensitive to inhibition by normal serum, and it had an unusual pattern of heat stability. Heating at 100 degrees C resulted in some recovery of activity of a sample previously inactivated by heating at 60 degrees C.

MeSH Terms
Hemagglutination Hemagglutinins/analysis Hot Temperature Hydroxamic Acids/pharmacology Peptide Hydrolases/analysis Solubility Vibrio cholerae/analysis,immunology
Chemicals
Hemagglutinins Hydroxamic Acids zincov Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Honda T
Booth B A
Boesman-Finkelstein M
Finkelstein R A
References (16)
16 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.2.451-454.1987
Published
1987-02-00
Pages
451-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260349
Subset
IM
Grants
NIAID NIH HHS · AI-17312 · United States
External Links
PubMed source DOI: 10.1128/iai.55.2.451-454.1987 PMC Full Text View journal details
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