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PMID: 6188694 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification and some properties of a non-o1 Vibrio cholerae enterotoxin that is identical to cholera enterotoxin.

Infection and immunity ·Vol. 39 ·No. 3 ·1983-03-00 ·Pages 1128-35

Yamamoto K, Takeda Y, Miwatani T, Craig JP

Abstract

Cholera-like enterotoxin was isolated and purified from the culture supernatant of a non-O1 strain of Vibrio cholerae, E8498, isolated from the environment. Enterotoxin was purified by aluminum hydroxide absorption and elution and successive gel filtrations on Sephadex G-100, Bio-Gel A-5m, and Sephadex G-75. Purified enterotoxin gave a single stained band on polyacrylamide gel disc electrophoresis, and the mobility was the same as that of cholera enterotoxin. The specific biological activity of the purified enterotoxin was almost the same as that of cholera enterotoxin in the Chinese hamster ovary cell assay, fluid accumulation in mouse ligated intestine, increase in vascular permeability in rabbit skin, and passive immune hemolysis. Sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis showed that the purified enterotoxin consisted of subunits A and B, identical to those of cholera enterotoxin, and Ouchterlony double gel diffusion tests indicated that the two toxins were immunologically identical. Enterotoxins prepared from several non-O1 strains isolated from human patients were also immunologically identical to cholera enterotoxin.

MeSH Terms
Biological Assay Cholera Toxin/immunology,isolation & purification,pharmacology Chromatography, Gel Electrophoresis, Polyacrylamide Gel Enterotoxins/immunology,isolation & purification,pharmacology Epitopes Serotyping Vibrio cholerae/analysis,classification
Chemicals
Enterotoxins Epitopes Cholera Toxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamamoto K
Takeda Y
Miwatani T
Craig J P
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.39.3.1128-1135.1983
Published
1983-03-00
Pages
1128-35
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC348073
Subset
IM
Grants
NIAID NIH HHS · 1 AI9602 · United States
External Links
PubMed source DOI: 10.1128/iai.39.3.1128-1135.1983 PMC Full Text View journal details
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