Home LiteratureArticle Details
PMID: 3554249 Published · ppublish English Journal Article

A segment of GCN4 mRNA containing the upstream AUG codons confers translational control upon a heterologous yeast transcript.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2863-7

Mueller PP, Harashima S, Hinnebusch AG

Abstract

GCN4 encodes a transcriptional activator in Saccharomyces cerevisiae that is regulated at the translational level. We show that an approximately 240-nucleotide segment from the GCN4 mRNA leader containing four AUG codons is sufficient to confer translational control typical of GCN4 upon a GAL1-lacZ fusion transcript. Regulation of the hybrid transcript is dependent upon multiple positive (GCN) and negative (GCD) trans-acting factors shown to regulate GCN4 expression post-transcriptionally. This result limits the target sequences for these factors to a small internal segment of the GCN4 mRNA leader. The elimination of AUG codons within this segment substantially reduces the usual derepressing effect of mutations in five GCD genes upon GCN4-lacZ expression. This supports the idea that the products of these negative regulatory genes act by modulating the effects of the upstream AUG codons on translation of GCN4 mRNA.

MeSH Terms
Base Sequence Codon Gene Expression Regulation Genes, Fungal Mutation RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Codon RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mueller P P
Harashima S
Hinnebusch A G
References (18)
18 references, click to expand
  1. Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae.
    J Mol Biol. 1975 Aug 5;96(2):273-90 PMID: 1100845
  2. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  3. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  4. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  5. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
    Nucleic Acids Res. 1982 Oct 25;10(20):6487-500 PMID: 6757864
  6. Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae.
    J Biol Chem. 1983 Apr 25;258(8):5238-47 PMID: 6300123
  7. Identification of AAS genes and their regulatory role in general control of amino acid biosynthesis in yeast.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2704-8 PMID: 6341997
  8. Positive regulation in the general amino acid control of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374-8 PMID: 6351059
  9. 5' untranslated sequences are required for the translational control of a yeast regulatory gene.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5096-100 PMID: 6433345
  10. Evidence for translational regulation of the activator of general amino acid control in yeast.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6442-6 PMID: 6387704
  11. Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Jul;4(7):1326-33 PMID: 6095062
  12. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  13. GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast.
    Cell. 1985 Nov;43(1):177-88 PMID: 3907851
  14. Multiple upstream AUG codons mediate translational control of GCN4.
    Cell. 1986 Apr 25;45(2):201-7 PMID: 3516411
  15. Multiple cis-acting elements modulate the translational efficiency of GCN4 mRNA in yeast.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4849-53 PMID: 3088566
  16. A hierarchy of trans-acting factors modulates translation of an activator of amino acid biosynthetic genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Sep;5(9):2349-60 PMID: 3915540
  17. Multiple GCD genes required for repression of GCN4, a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Nov;6(11):3990-8 PMID: 3540603
  18. Identification and characterization of four new GCD genes in Saccharomyces cerevisiae.
    Curr Genet. 1986;10(9):657-64 PMID: 3329041
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2863
Published
1987-05-00
Pages
2863-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304760
Subset
IM
Databases
GENBANK
M16269, M16270
External Links
PubMed source DOI: 10.1073/pnas.84.9.2863 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2