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PMID: 3566240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Novel type of plasmid-borne resistance to trimethoprim.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 1 ·1987-01-00 ·Pages 60-6

Sundström L, Vinayagamoorthy T, Sköld O

Abstract

A novel trait for transferable resistance to high concentrations of trimethoprim was found to dominate among enterobacteria collected from different parts of Sri Lanka. Drug resistance was a result of the production of dihydrofolate reductase with a decreased sensitivity to antifolates. By characterization of the partially purified enzyme and by restriction enzyme digestion analysis, the newly found gene was shown to be distinct from the earlier known plasmid-borne resistance genes which express dihydrofolate reductases of types I, II, and III. Cloning of fragments containing the resistance gene and further restriction enzyme digestion analysis showed that this gene was inserted very close to a sulfonamide resistance gene. Evolution of trimethoprim resistance in Sri Lanka thus seems to have taken a different route from that taken in the industrialized world, where transposon Tn7 seems to dominate. The close combination of the new trimethoprim resistance gene with sulfonamide resistance on the plasmids studied would effect an efficient spread of these genes, since trimethoprim has most often been used in combination with a sulfonamide.

MeSH Terms
Chromatography, Ion Exchange Conjugation, Genetic DNA, Bacterial/analysis Drug Resistance, Microbial Electrophoresis, Agar Gel Enterobacteriaceae/drug effects,genetics Genes, Bacterial Nucleic Acid Hybridization R Factors Sri Lanka Trimethoprim/pharmacology
Chemicals
DNA, Bacterial Trimethoprim
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sundström L
Vinayagamoorthy T
Sköld O
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31 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.1.60
Published
1987-01-00
Pages
60-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC174652
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.1.60 PMC Full Text View journal details
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