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PMID: 3638932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alterations in kinetic properties of penicillin-binding proteins of penicillin-resistant Streptococcus pneumoniae.

Antimicrobial agents and chemotherapy ·Vol. 30 ·No. 1 ·1986-07-00 ·Pages 57-63

Handwerger S, Tomasz A

Abstract

Earlier studies have shown that the highly penicillin-resistant South African Strains of pneumococci contain altered penicillin-binding proteins (PBPs) (S. Zighelboim and A. Tomasz, Antimicrob. Agents Chemother. 17:434-442, 1980). We now describe a detailed quantitative characterization of the reaction of radioactively labeled penicillin with the PBPs of the penicillin-susceptible and penicillin-resistant pneumococci and several intermediate-resistance-level genetic transformants as well. The altered binding of the antibiotic by the PBPs of resistant cells appears to be due to a combination of two factors: lower drug affinity and change in the cellular amounts of PBPs. No alteration in the rates of deacylation of the penicilloyl-PBPs of the resistant cells was detected.

MeSH Terms
Bacterial Proteins Carboxypeptidases/metabolism Carrier Proteins/metabolism Dealkylation Electrophoresis, Polyacrylamide Gel Hexosyltransferases Kinetics Muramoylpentapeptide Carboxypeptidase/metabolism Penicillin Resistance Penicillin-Binding Proteins Penicillins/pharmacology Peptidyl Transferases Streptococcus pneumoniae/drug effects,metabolism
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Penicillins Peptidyl Transferases Hexosyltransferases Carboxypeptidases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Handwerger S
Tomasz A
References (23)
23 references, click to expand
  1. Escherichia coli resistance to beta-lactam antibiotics through a decrease in the affinity of a target for lethality.
    Nature. 1978 Aug 17;274(5672):713-5 PMID: 209344
  2. A study of the genetic material determining an enzyme in Pneumococcus.
    Biochim Biophys Acta. 1960 Apr 22;39:508-18 PMID: 14413322
  3. Penicillin-binding components of penicillin-susceptible and -resistant strains of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1980 Sep;18(3):390-6 PMID: 6903437
  4. Bacillus megaterium resistance to cloxacillin accompanied by a compensatory change in penicillin binding proteins.
    Nature. 1979 Jul 12;280(5718):167-8 PMID: 121895
  5. Penicillin-binding proteins of penicillin-susceptible and intrinsically resistant Neisseria gonorrhoeae.
    Antimicrob Agents Chemother. 1980 Nov;18(5):730-7 PMID: 6778384
  6. Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus.
    FEBS Lett. 1980 Dec 29;122(2):275-8 PMID: 7202719
  7. The role of penicillin-proteins in the action of cephalosporins against Escherichia coli and Salmonella typhimurium.
    Eur J Biochem. 1981 Jul;117(2):301-10 PMID: 7023942
  8. beta-Lactam-resistant Pseudomonas aeruginosa with modified penicillin-binding proteins emerging during cystic fibrosis treatment.
    Antimicrob Agents Chemother. 1981 May;19(5):705-11 PMID: 6457554
  9. Physiological properties of penicillin-binding proteins in group A streptococci.
    Antimicrob Agents Chemother. 1981 May;19(5):872-80 PMID: 7027926
  10. Penicillin-resistant and penicillin-tolerant mutants of group A Streptococci.
    Antimicrob Agents Chemother. 1982 Jul;22(1):128-36 PMID: 6181734
  11. Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1982 Jul;22(1):172-5 PMID: 7125630
  12. Purification of penicillin-binding protein 3 from Streptococcus pneumoniae.
    Eur J Biochem. 1982 Oct;127(2):231-6 PMID: 7140764
  13. Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin.
    J Bacteriol. 1983 Sep;155(3):1343-50 PMID: 6411688
  14. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.
    J Bacteriol. 1984 May;158(2):513-6 PMID: 6563036
  15. Characterization of non-beta-lactamase-mediated ampicillin resistance in Haemophilus influenzae.
    Antimicrob Agents Chemother. 1984 Aug;26(2):235-44 PMID: 6333208
  16. Penicillin-binding proteins of Streptococcus pneumoniae: characterization of tryptic peptides containing the beta-lactam-binding site.
    Eur J Biochem. 1984 Nov 2;144(3):637-41 PMID: 6489345
  17. The penicillin receptor in Streptomyces.
    Ann N Y Acad Sci. 1974 May 10;235(0):236-68 PMID: 4528051
  18. Altered penicillin-binding components in penicillin-resistant mutants of Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1816-20 PMID: 819927
  19. Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.
    Biochem J. 1976 Jun 1;155(3):623-9 PMID: 949323
  20. Secretion of lipids induced by inhibition of peptidoglycan synthesis in streptococci.
    J Bacteriol. 1977 Nov;132(2):704-17 PMID: 21168
  21. Alterations in penicillin-binding proteins of clinical and laboratory isolates of pathogenic Streptococcus pneumoniae with low levels of penicillin resistance.
    J Infect Dis. 1986 Jan;153(1):83-9 PMID: 3941290
  22. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  23. Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1980 Mar;17(3):434-42 PMID: 6903436
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.30.1.57
Published
1986-07-00
Pages
57-63
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC176435
Subset
IM
Grants
NIAID NIH HHS · AI 16794 · United States
External Links
PubMed source DOI: 10.1128/AAC.30.1.57 PMC Full Text View journal details
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