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PMID: 3640338 Published · ppublish English Journal Article

Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 7883-96

Stetler G, Brewer MT, Thompson RC

Abstract

We report the isolation of the human gene encoding an inhibitor of neutrophil elastase and cathepsin G. We have sequenced the gene and a cDNA clone isolated from human parotid tissue. The protein encoded by this gene appears to contain two functional domains, one having a trypsin inhibitory site and the other an elastase inhibitory site. The two-domain structure of the protein is reflected in the organization of the gene, with each domain represented by a separate exon. We have also noted that the intervening sequence separating the trypsin-inhibitor-exon and the elastase-inhibitor-exon is flanked by eleven base-pair direct repeats, suggesting that this intron may have been generated by a transposition-type event.

MeSH Terms
Base Sequence Cathepsin G Cathepsins/antagonists & inhibitors Exons Genes Humans Introns Leukocytes/enzymology Oligonucleotides/chemical synthesis,genetics Pancreatic Elastase/antagonists & inhibitors Parotid Gland/physiology Protease Inhibitors/genetics,metabolism Protein Conformation Serine Endopeptidases Structure-Activity Relationship
Chemicals
Oligonucleotides Protease Inhibitors Cathepsins Serine Endopeptidases CTSG protein, human Cathepsin G Pancreatic Elastase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stetler G
Brewer M T
Thompson R C
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.20.7883
Published
1986-10-24
Pages
7883-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311822
Subset
IM
Databases
GENBANK
X04502, X04503
External Links
PubMed source DOI: 10.1093/nar/14.20.7883 PMC Full Text View journal details
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