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PMID: 3647358 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Natural point mutations within rat rDNA transcription terminator elements reveal the functional importance of single bases for factor binding and termination.

Nucleic acids research ·Vol. 15 ·No. 10 ·1987-05-26 ·Pages 4131-43

Kermekchiev MB, Grummt I

Abstract

The rat rDNA transcription unit extends 560-565 bp into the spacer downstream of the 28S rRNA coding region. The site of 3' end formation is located in front of a conserved 18 bp sequence element which is repeated eight times in the 3' spacer between nucleotides +582 and +1767 relative to the 3' terminus of 28S rRNA. These sequence motifs are almost identical to the RNA polymerase I transcription termination signal (the Sal I box) that has previously been identified in the 3' terminal spacer of mouse rDNA. Interestingly, each of the single rat elements contains one or more base substitutions as compared to the murine Sal I box. Individual rat Sal I boxes were cloned and tested for their ability to interact with the murine termination factor and to direct transcription termination. It is shown that five of the eight boxes represent genuine transcription terminators, while three elements contain certain point mutations which are not recognized by the nuclear Sal I box-binding protein and therefore are functionally inactive.Images

MeSH Terms

Animals Base Sequence DNA, Ribosomal/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation Genes, Regulator Mice/genetics Mutation Nucleic Acid Precursors/biosynthesis RNA Polymerase I/metabolism RNA Precursors RNA, Ribosomal/biosynthesis Rats/genetics Sequence Homology, Nucleic Acid Species Specificity Terminator Regions, Genetic Transcription Factors/metabolism Transcription, Genetic

Chemicals

DNA, Ribosomal DNA-Binding Proteins Nucleic Acid Precursors RNA Precursors RNA, Ribosomal Transcription Factors RNA Polymerase I

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kermekchiev M B

Grummt I

References (23)

23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/15.10.4131

Published

1987-05-26

Pages

4131-43

Language

English

Region

England

NLM ID

0411011

PMCID

PMC340837

Subset

External Links

PubMed source DOI: 10.1093/nar/15.10.4131 PMC Full Text View journal details

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Natural point mutations within rat rDNA transcription terminator elements reveal the functional importance of single bases for factor binding and termination.

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