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PMID: 3960734 Published · ppublish English Journal Article

Nucleotide sequence analysis of the spacer regions flanking the rat rRNA transcription unit and identification of repetitive elements.

Nucleic acids research ·Vol. 14 ·No. 6 ·1986-03-25 ·Pages 2799-810

Yavachev LP, Georgiev OI, Braga EA, Avdonina TA, Bogomolova AE, Zhurkin VB, Nosikov VV, Hadjiolov AA

Abstract

We investigated the organization of the rat rDNA non-transcribed spacer (NTS) by determining the sequence of large NTS segments located upstream (2501 bp) and downstream (4025 bp) from the rRNA transcription unit. We identified four B2-like and two ID mobile elements. They may be grouped in three pairs with the members of each pair located in the upstream and downstream NTS. The ID sequences are identical to the consensus sequence, while the pairs of B2-like elements show 85% and 50/65% homology to the consensus B2 sequence. The proximal part of the downstream NTS contains a region of widely diverged SalI tandem repeats. A considerable part of the analyzed upstream and downstream NTS sequences is constituted by different types of simple sequences and long poly(purine) X poly(pyrimidine) tracts. These data show that the rat rDNA NTS regions flanking the rRNA transcription unit are characterized by a combination of short interspersed (B2-superfamily) and various simple sequences.

MeSH Terms
Animals Base Sequence DNA, Ribosomal/genetics Genetic Linkage RNA, Ribosomal/genetics Rats Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yavachev L P
Georgiev O I
Braga E A
Avdonina T A
Bogomolova A E
Zhurkin V B
Nosikov V V
Hadjiolov A A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.6.2799
Published
1986-03-25
Pages
2799-810
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339699
Subset
IM
Databases
GENBANK
X03694, X03695
External Links
PubMed source DOI: 10.1093/nar/14.6.2799 PMC Full Text View journal details
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