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PMID: 3676438 Published · ppublish English Journal Article

Activity-dependent calcium transients in central nervous system myelinated axons revealed by the calcium indicator Fura-2.

Biophysical journal ·Vol. 52 ·No. 4 ·1987-10-00 ·Pages 571-6

Lev-Ram V, Grinvald A

Abstract

Optical measurements from rat optic nerve, loaded with the new Ca2+ indicator Fura-2, provide the first evidence for the presence of activity-dependent fast intracellular [Ca2+] transients in mammalian central nervous system (CNS) myelinated axons. The results suggest that voltage-dependent Ca2+ channels are present in some of the myelinated axons. Optical measurements from axons stained with anterogradely transported voltage-sensitive dye suggest the presence of Ca2+-dependent potassium conductances in these axons. This report also demonstrates that Fura-2 can readily detect changes in [Ca2+] inside cells as a result of electrical activity, and establishes its suitability for measurements of intracellular Ca2+ transients in the millisecond time domain.

MeSH Terms
Animals Axons/physiology Benzofurans Calcium/physiology Fluorescent Dyes Fura-2 In Vitro Techniques Membrane Potentials Nerve Fibers, Myelinated/physiology Neural Conduction Optic Nerve/physiology Rats Rats, Inbred Strains
Chemicals
Benzofurans Fluorescent Dyes Calcium Fura-2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lev-Ram V
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Grinvald A
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(87)83246-0
Published
1987-10-00
Pages
571-6
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330047
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(87)83246-0 PMC Full Text View journal details
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