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PMID: 3680173 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region.

Journal of bacteriology ·Vol. 169 ·No. 12 ·1987-12-00 ·Pages 5653-62

Conway T, Sewell GW, Ingram LO

Abstract

The gene encoding glyceraldehyde-3-phosphate dehydrogenase was isolated from a library of Zymomonas mobilis DNA fragments by complementing a deficient strain of Escherichia coli. It contained tandem promoters which were recognized by E. coli but appeared to function less efficiently than the enteric lac promoter in E. coli. The open reading frame for this gene encoded 337 amino acids with an aggregate molecular weight of 36,099 (including the N-terminal methionine). The primary amino acid sequence for this gene had considerable functional homology and amino acid identity with other eucaryotic and bacterial genes. Based on this comparison, the gap gene from Z. mobilis appeared to be most closely related to that of the thermophilic bacteria and to the chloroplast isozymes. Comparison of this gene with other glycolytic enzymes from Z. mobilis revealed a conserved pattern of codon bias and several common features of gene structure. A tentative transcriptional consensus sequence is proposed for Z. mobilis based on comparison of the five known promoters for three glycolytic enzymes.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon/genetics DNA, Bacterial/genetics Genes, Bacterial Glyceraldehyde-3-Phosphate Dehydrogenases/genetics Gram-Negative Bacteria/enzymology,genetics Molecular Sequence Data Promoter Regions, Genetic Transcription, Genetic
Chemicals
Codon DNA, Bacterial Glyceraldehyde-3-Phosphate Dehydrogenases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Conway T
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Sewell G W
Ingram L O
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.12.5653-5662.1987
Published
1987-12-00
Pages
5653-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214028
Subset
IM
Databases
GENBANK
M18802
External Links
PubMed source DOI: 10.1128/jb.169.12.5653-5662.1987 PMC Full Text View journal details
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