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PMID: 3691479 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Novel amber suppressor tRNAs of mammalian origin.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 3049-55

Valle RP, Morch MD, Haenni AL

Abstract

Two amber suppressor tRNAs have been isolated from calf liver. They are different from previously identified naturally occurring amber suppressors of eukaryotes in so far as they are neither tRNATyr nor tRNAGln. They are leucine iso-acceptors and their nucleotide sequence indicates that they harbour a CAA and a CAG anticodon respectively. Both species are functional as amber suppressors as demonstrated by readthrough of the amber codon which terminates the 126 kd protein gene of tobacco mosaic virus RNA. The results bring new information in the discussion of codon-anticodon recognition and regulation of termination in eukaryotic protein synthesis.

MeSH Terms
Animals Base Sequence Cattle Liver/metabolism Molecular Sequence Data Nucleic Acid Conformation Protein Biosynthesis RNA, Transfer/genetics,isolation & purification,metabolism RNA, Transfer, Glu/genetics RNA, Transfer, Tyr/genetics Suppression, Genetic Tyrosine/metabolism
Chemicals
RNA, Transfer, Glu RNA, Transfer, Tyr Tyrosine RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Valle R P
Institut Jacques Monod, Paris, France.
Morch M D
Haenni A L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
3049-55
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553742
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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