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PMID: 372759 Published · ppublish English Journal Article

A streptomycin-resistant Escherichia coli mutant with ribosomes temperature-sensitive in the suppression of a nonsense codon.

Molecular & general genetics : MGG ·Vol. 170 ·No. 2 ·1979-02-26 ·Pages 149-53

Zeevi M, Daniel V, Engelberg-Kulka H

Abstract

Cell free extracts from a streptomycin-resistant E. coli mutant which is also temperature-sensitive for Q beta phage were studied for suppression of a nonsense mutation at various temperatures. The streptomycin-resistant ribosomes of the mutant were found to be temperature-sensitive in suppression of an amber mutation in f2 phage coat protein while retaining the ability to synthesize proteins at an elevated temperature (42 degrees C). The restriction of amber suppression at 42 degrees C is assumed to be related to an alteration in ribosomal protein S12 of the streptomycin-resistant mutant which also causes a change in its electrophoretic mobility.

MeSH Terms
Cell-Free System Drug Resistance, Microbial Escherichia coli/genetics,metabolism Genetic Code Hot Temperature Mutation Peptide Chain Termination, Translational Phenotype Ribosomes/metabolism Streptomycin/pharmacology Suppression, Genetic
Chemicals
Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zeevi M
Daniel V
Engelberg-Kulka H
References (25)
25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337790
Published
1979-02-26
Pages
149-53
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337790
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