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PMID: 372760 Published · ppublish English Journal Article

The requirement of nonsense suppression for the development of several phages.

Molecular & general genetics : MGG ·Vol. 170 ·No. 2 ·1979-02-26 ·Pages 155-9

Engelberg-Kulka H, Dekel L, Israeli-Reches M, Belfort M

Abstract

A spontaneous streptomycin-resistant Escherichia coli mutant which is temperature-sensitive for suppression of a nonsense codon was studied for its ability to propagate phages T2, T4D, T5, phi K, f2, MS2, R17, Q beta, lambda as well as filamentous phages fl, fd and M13. Of all phages tested, only the growth of Q beta, lambda, and filamentous phages is inhibited in the mutant at 42 degree C. This selective inhibition suggests that, like Q beta, lambda and filamentous phages also require a read-through proten(s) which results from suppression of a termination codon.

MeSH Terms
Coliphages/growth & development Drug Resistance, Microbial Escherichia coli/genetics Genetic Code Lysogeny Mutation Peptide Chain Termination, Translational Phenotype Streptomycin/pharmacology Suppression, Genetic
Chemicals
Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Engelberg-Kulka H
Dekel L
Israeli-Reches M
Belfort M
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337791
Published
1979-02-26
Pages
155-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337791
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