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PMID: 3741986 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Optical imaging of cell membrane potential changes induced by applied electric fields.

Biophysical journal ·Vol. 50 ·No. 2 ·1986-08-00 ·Pages 339-48

Gross D, Loew LM, Webb WW

Abstract

We report the first imaging of the spatial distributions of transmembrane potential changes induced in nonexcitable cells by applied external electric fields. These changes are indicated by the fluorescence intensity of a charge-shift potentiometric dye incorporated in the cell plasma membrane and measured by digital intensified video microscopy.

MeSH Terms
Basidiomycota/physiology Carcinoma, Squamous Cell Cell Line Cell Membrane/physiology Electrophysiology/methods Fluorescent Dyes Humans Membrane Potentials Plant Physiological Phenomena Protoplasts/physiology Pyridinium Compounds Secale/physiology
Chemicals
Fluorescent Dyes Pyridinium Compounds 1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gross D
Loew L M
Webb W W
References (38)
38 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(86)83467-1
Published
1986-08-00
Pages
339-48
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1329750
Subset
IM
Grants
NCI NIH HHS · CA00677 · United States
NIGMS NIH HHS · GM25190 · United States
NIGMS NIH HHS · GM35063 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(86)83467-1 PMC Full Text View journal details
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