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PMID: 3827851 Published · ppublish English Journal Article

Specificity of inositol phosphate-stimulated Ca2+ mobilization from Swiss-mouse 3T3 cells.

The Biochemical journal ·Vol. 240 ·No. 1 ·1986-11-15 ·Pages 301-4

Irvine RF, Letcher AJ, Lander DJ, Berridge MJ

Abstract

Pure samples of inositol 1,3,4-trisphosphate, inositol 1,3,4,5-tetrakisphosphate and inositol 1,2-cyclic 4,5-trisphosphate were prepared and tested for their ability to mobilize calcium from intracellular stores in a permeabilized Swiss mouse 3T3 cell preparation. In this system inositol 1,4,5-trisphosphate mobilizes Ca2+ with a half-maximal dose of 0.3 microM. Inositol 1,2-cyclic 4,5-trisphosphate mobilized Ca2+ to the same extent with a half-maximal dose of 0.3 microM, whereas inositol 1,3,4-trisphosphate required a half-maximal dose of approx. 9 microM to give the same effect. Inositol 1,3,4,5-tetrakisphosphate was ineffective up to 20 microM and at that concentration did not antagonize the mobilization induced by inositol 1,4,5-trisphosphate. The relevance of these findings to the function of the inositol tris/tetrakis-phosphate pathway is discussed.

MeSH Terms
Animals Calcium/metabolism Cell Line Dose-Response Relationship, Drug Fibroblasts/drug effects,metabolism Inositol Phosphates/pharmacology Mice Mice, Inbred Strains Sugar Phosphates/pharmacology
Chemicals
Inositol Phosphates Sugar Phosphates Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Irvine R F
Letcher A J
Lander D J
Berridge M J
References (26)
26 references, click to expand
  1. Isolation and separation of inositol phosphates from hydrolysates of rat tissues.
    Biochim Biophys Acta. 1965 Jun 1;98(3):574-81 PMID: 5837456
  2. Myo-inositol phosphates obtained by alkaline hydrolysis of beef brain phosphoinositide.
    J Biol Chem. 1961 Jan;236:54-60 PMID: 13707646
  3. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.
    Lipids. 1970 May;5(5):494-6 PMID: 5483450
  4. Studies on the interactions of nucleotides, polynucleotides, and nucleic acids with dihydroxyboryl-substituted celluloses.
    Biochemistry. 1972 Sep 12;11(19):3623-8 PMID: 5053763
  5. D-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase.
    Biochem J. 1972 Mar;127(1):113-8 PMID: 4342209
  6. Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids.
    Biochem J. 1981 Apr 1;195(1):301-6 PMID: 7306057
  7. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane.
    Biochem J. 1982 Apr 1;203(1):169-77 PMID: 6285891
  8. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.
    Biochem J. 1983 May 15;212(2):473-82 PMID: 6309146
  9. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
    Nature. 1983 Nov 3-9;306(5938):67-9 PMID: 6605482
  10. The second messenger linking receptor activation to internal Ca release in liver.
    Nature. 1984 May 3-9;309(5963):63-6 PMID: 6325926
  11. Inositol trisphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor.
    Biochem J. 1984 Aug 15;222(1):195-201 PMID: 6089758
  12. Specificity of inositol trisphosphate-induced calcium release from permeabilized Swiss-mouse 3T3 cells.
    Biochem J. 1984 Aug 15;222(1):269-72 PMID: 6332622
  13. Inositol trisphosphates in carbachol-stimulated rat parotid glands.
    Biochem J. 1984 Oct 1;223(1):237-43 PMID: 6333870
  14. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  15. Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes.
    Biochem J. 1984 Dec 15;224(3):741-6 PMID: 6525174
  16. Inositol cyclic phosphates are produced by cleavage of phosphatidylphosphoinositols (polyphosphoinositides) with purified sheep seminal vesicle phospholipase C enzymes.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4013-7 PMID: 2987959
  17. Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands.
    Biochem J. 1985 Jul 15;229(2):505-11 PMID: 2994638
  18. Isolation and characterization of the inositol cyclic phosphate products of polyphosphoinositide cleavage by phospholipase C. Physiological effects in permeabilized platelets and Limulus photoreceptor cells.
    J Biol Chem. 1985 Nov 5;260(25):13496-501 PMID: 2997167
  19. Isolation and characterization of the inositol cyclic phosphate products of phosphoinositide cleavage by phospholipase C. Metabolism in cell-free extracts.
    J Biol Chem. 1986 Jan 5;261(1):122-6 PMID: 3001044
  20. Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.
    Biochem J. 1985 Nov 15;232(1):211-5 PMID: 4084229
  21. Inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate formation in Ca2+-mobilizing-hormone-activated cells.
    Biochem J. 1985 Nov 15;232(1):237-43 PMID: 3002326
  22. The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues.
    Nature. 1986 Apr 17-23;320(6063):631-4 PMID: 3010126
  23. Cyclic guanosine monophosphate inhibition of contraction may be mediated through inhibition of phosphatidylinositol hydrolysis in rat aorta.
    Circ Res. 1986 Mar;58(3):407-10 PMID: 3013461
  24. Second messenger function of inositol 1,4,5-trisphosphate. Early changes in inositol phosphates, cytosolic Ca2+, and insulin release in carbamylcholine-stimulated RINm5F cells.
    J Biol Chem. 1986 Jun 25;261(18):8314-9 PMID: 3522567
  25. Glucose-induced accumulation of inositol trisphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer.
    Biochem J. 1986 Jul 1;237(1):259-63 PMID: 3541896
  26. The structure of triphosphoinositide from beef brain.
    Biochim Biophys Acta. 1966 Dec 7;125(3):413-21 PMID: 4291568
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2400301
Published
1986-11-15
Pages
301-4
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147412
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2400301 PMC Full Text View journal details
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