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PMID: 3839073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes.

Nucleic acids research ·Vol. 13 ·No. 1 ·1985-01-11 ·Pages 239-49

Mauro VP, Nguyen T, Katinakis P, Verma DP

Abstract

The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin-23 gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic clone indicated that the coding region of this gene is 669 bp long and is interrupted by a single intron of about 530 bp. The deduced protein sequence suggests that nodulin-23 may have a signal sequence. The 5'-flanking sequence of two other nodulin genes, nodulin-24 encoding for a membrane polypeptide and one of the leghemoglobin genes (LbC3), were obtained. Comparison of these sequences revealed three conserved regions, one of which, an octanucleotide (GTTTCCCT), has 100% homology. The conserved sequences are arranged in a unique fashion and have a spatial organization with respect to order and position, which may suggest a potential regulatory role in controlling the expression of nodulin and leghemoglobin genes during symbiosis.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics Gene Expression Regulation Genes Hemeproteins/genetics Leghemoglobin/genetics Membrane Proteins Operon Plant Proteins/genetics Rhizobium/genetics Soybeans/genetics Transcription, Genetic
Chemicals
Hemeproteins Leghemoglobin Membrane Proteins Plant Proteins nodulin DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mauro V P
Nguyen T
Katinakis P
Verma D P
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.1.239
Published
1985-01-11
Pages
239-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340987
Subset
IM
Databases
GENBANK
X01704, X02726, X02727
External Links
PubMed source DOI: 10.1093/nar/13.1.239 PMC Full Text View journal details
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