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PMID: 3859867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4351-5

Furneaux HM, Perkins KK, Freyer GA, Arenas J, Hurwitz J

Abstract

A nuclear extract from HeLa cells has been separated by DEAE-cellulose chromatography into two fractions, both of which are required for mRNA splicing in vitro. Both fractions are heat labile and sensitive to N-ethylmaleimide. The activity of one of the fractions was abolished by preincubation with micrococcal nuclease, while the other fraction was unaffected by this treatment. This abolition indicates an essential nucleic acid component. Fractions I and II are required for the in vitro splicing of human beta-globin and adenovirus transcripts.

MeSH Terms

Chromatography, DEAE-Cellulose Ethylmaleimide/pharmacology HeLa Cells/analysis Hot Temperature Humans Micrococcal Nuclease/metabolism RNA Splicing RNA, Messenger/isolation & purification Transcription, Genetic

Chemicals

RNA, Messenger Micrococcal Nuclease Ethylmaleimide

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Furneaux H M

Perkins K K

Freyer G A

Arenas J

Hurwitz J

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.13.4351

Published

1985-07-00

Pages

4351-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC390411

Subset

Grants

NCI NIH HHS · CA 13106 · United States

External Links

PubMed source DOI: 10.1073/pnas.82.13.4351 PMC Full Text View journal details

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Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

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