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PMID: 3884972 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physiology of the SOS response: kinetics of lexA and recA transcriptional activity following induction.

Molecular & general genetics : MGG ·Vol. 198 ·No. 2 ·1985-00-00 ·Pages 207-12

Markham BE, Harper JE, Mount DW

Abstract

The products of the lexA and recA genes play central roles in the regulation of the Escherichia coli SOS response. We have measured the rate of mRNA synthesis from each gene at intervals following various inducing treatments in order to obtain a more precise timing of the induction process. Further, we provide quantitative evidence for kinetics of decay from fully induced levels of mRNA synthesis to basal levels as the cells shut down the SOS response which are in agreement with previously published data on the expression of specific SOS functions. The induction kinetics of lexA and recA gene expression are parallel except for nalidixic acid (NAL) treatment, with the actual levels of lexA mRNA synthesis being about 10-fold lower than that of recA. Reestablishment of repression from RecA commenced over 30 min earlier than from lexA. These results are fully consistent with the model that the functions result from the increased gene expression.

MeSH Terms
Bacterial Proteins/genetics DNA Repair/drug effects,radiation effects Dose-Response Relationship, Radiation Escherichia coli/genetics Genes, Bacterial Nalidixic Acid/pharmacology Rec A Recombinases/genetics Repressor Proteins/genetics Serine Endopeptidases Transcription Factors/genetics Transcription, Genetic/drug effects,radiation effects Ultraviolet Rays
Chemicals
Bacterial Proteins LexA protein, Bacteria Repressor Proteins Transcription Factors Nalidixic Acid Rec A Recombinases Serine Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Markham B E
Harper J E
Mount D W
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45 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00382997
Published
1985-00-00
Pages
207-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM24496 · United States
External Links
PubMed source DOI: 10.1007/BF00382997
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