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PMID: 3889840 Published · ppublish English Journal Article

Does the 'non-coding' strand code?

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1389-97

Sharp PM

Abstract

The hypothesis that DNA strands complementary to the coding strand contain in phase coding sequences has been investigated. Statistical analysis of the 50 genes of bacteriophage T7 shows no significant correlation between patterns of codon usage on the coding and non-coding strands. In Bacillus and yeast genes the correlation observed is not different from that expected with random synonymous codon usage, while a high correlation seen in 52 E. coli genes can be explained in terms of an excess of RNY codons. A deficiency of UUA, CUA and UCA codons (complementary to termination) seems to be restricted to the E. coli genes, and may be due to low abundance of the relevant cognate tRNA species. Thus the analysis shows that the non-coding strand has the properties expected of a sequence complementary to a coding strand, with no indications that it encodes, or may have encoded, proteins.

MeSH Terms
Bacillus/genetics Base Sequence Codon DNA/genetics DNA, Bacterial/genetics DNA, Fungal/genetics DNA, Viral/genetics Genes Genes, Bacterial Genes, Fungal Genes, Viral RNA, Messenger/genetics Saccharomyces cerevisiae/genetics T-Phages/genetics Templates, Genetic
Chemicals
Codon DNA, Bacterial DNA, Fungal DNA, Viral RNA, Messenger DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Sharp P M
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19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1389
Published
1985-02-25
Pages
1389-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341079
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.4.1389 PMC Full Text View journal details
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